Main Article Content

Endah Prayekti Suliati Suliati Dwi Agustin Wulandari

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.

Downloads

Download data is not yet available.

Article Details

How to Cite
Prayekti, E., Suliati, S. and Wulandari, D. A. (2021) “Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli”, Indonesian Journal of Medical Laboratory Science and Technology, 3(1), pp. 19–25. doi: 10.33086/ijmlst.v3i1.1906.
Section
Articles
Coconut Water, Escherichia coli, Mac Conkey

References

Directorate General of Estate Crops, Tree Crop Estate Stattistic of Indonesia 2015-2017. Jakarta: Secretariate of Directorate General of Estate Crops, Directorate General of Estate Crops, Ministry of Agriculture, 2016.

Yong JWH, Ge L, Ng YF, Tan SN, The chemical composition and biological properties of coconut (Cocos Nucifera L.) water. Molecules. 2009;14(12):5144–5164. doi: 10.3390/molecules14125144.

Appaiah P, Sunil L, Kumar PKP, Krishna AGG. Physico-chemical characteristics and stability aspects of coconut water and kernel at different stages of maturity. J Food Sci Technol. 2015;52(8):5196–5203 doi: 10.1007/s13197-014-1559-4.

DebMandal M, Mandal S. Coconut (Cocos nucifera L.: Arecaceae): In health promotion and disease prevention. Asian Pac. J Trop Med. 2011;4(3): 241–247. doi: 10.1016/S1995-7645(11)60078-3.

Zulaikhah S. Health benefits of tender coconut water (TCW). Int J Pharm Sci Res. 2019; 10(2).474-8 doi: 10.13040/IJPSR.0975-8232.10(2).474-8.

Halim HH, Dee EW, Dek MSP, Hamid AA, Ngalim A, Saari N, Jaafar AH. Ergogenic attributes of young and mature coconut (Cocos nucifera L.) water based on physical properties, sugars and electrolytes contents. Int J Food Prop. 2018;21(1):2378–2389. doi: 10.1080/10942912.2018.1522329.

Gayathry G. Production of nata de coco - a natural dietary fibre product from mature coconut water using Gluconacetobacter xylinum (sju-1). Int J Food Ferment Technol. 2015;5:231 doi: 10.5958/2277-9396.2016.00006.4.

Thankappan G, Anitha P. Effect of sources of coconut water and acidulants on physico chemical properties of nata-de-coco. J Trop Agric. 2018;56(2):206–209.

Yang SC, Lin CH, Aljuffali IA, Fang JY. Current pathogenic Escherichia coli foodborne outbreak cases and therapy development. Arch Microbiol. 2017;199(6):811-825. doi: 10.1007/s00203-017-1393-y.

Zhou Y, Zhu X, Hongyan H, Lu Y, Lu J, Mau L, Mau L, Sun Z. Characteristics of diarrheagenic Escherichia coli among children under 5 years of age with acute diarrhea: a hospital based study. BMC Infect Dis. 2018;18(63):1-10. doi: 10.1186/s12879-017-2936-1.

Jiang ZD, Dupont HL. Etiology of travellers’ diarrhea. Int Soc Travel Med. 2017;24(1):13-16. doi: 10.1093/jtm/tax003.

Sasakova N, Gregova G, Takacova D, Mojzisova J, Papajova I, Venglovsky J, Szaboova T, Kovacova S. Pollution of surface and ground water by sources related to agricultural activities. Front Sustain Food Syst. 2018;2:42. doi: 10.3389/fsufs.2018.00042.

Jang J, Hur HG, Sadowsky MJ, Byappanahalli MN, Yan T, Ishii S. Environmental Escherichia coli: ecology and public health implications—a review. J Appl Microbiol. 2017;123(3):570–581. doi: 10.1111/jam.13468.

Ercumen A, Pickering AJ, Kwong LH, Arnold BF, Parvez SM, Alam M, Sen D, Islam S, Kullmann C, Chase C, Ahmed R, Unicomb L, Luby SP, Jr JMC. Animal feces contribute to domestic fecal contamination: evidence from E. coli measured in water, hands, food, flies, and soil in Bangladesh. 2017. doi: 10.1021/acs.est.7b01710.

Prades A, Dornier M, Diop N, Pain JP. Coconut water uses, composition and properties: A review. Fruits. 2012;67(2):87-107. doi: 10.1051/fruits/2012002.

Chuku LC, Kalagbor GI. Protein and mineral element content of coconut (Cocos nucifera) water from different species. Am J Adv Drug Deliv. 2014;2(4):451-457. [Online]. Available: www.ajadd.co.uk.

Bren A, Park JO, Towbin BD, Dekel E, Rabinowitz JD, Alon U. Glucose becomes one of the worst carbon sources for E. coli on poor nitrogen sources due to suboptimal levels of cAMP. Sci Rep. 2016:2-11. doi: 10.1038/srep24834.

Li SHJ, Li Z, Park JO, King CG, Rabinowtiz JD, Wingreen NS, Gitai Z. Carbon, nitrogen and phosphorus limitation conditions. Nat Microbiol. 2018;3(8):939-947. doi: 10.1038/s41564-018-0199-2.Escherichia.

Husin N, Kamal SMM, Chuan LT, Muhammad NF, Jusoh N. Comparison of microbial growth on fish waste peptones from different hydrolysis methods, 2015 5th Int Conf Biomed Eng Technol (ICBET 2015). 2015;81(1):54-57. doi: 10.7763/IPCBEE.

Mulyanto A, Mujahid I, Khasanah TU. Kemampuan air kelapa muda sebagai antimikroba terhadap bakteri Escherichia coli penyebab diare [The ability of young coconut water as an antimicrobial against the bacteria Escherichia coli that causes diarrhea]. J Bio-Site. 2018;(4)1:18.

Endah Prayekti, Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia

Suliati Suliati, Department of Medical Laboratory Technology, Poltekkes Surabaya, Surabaya, Indonesia

Dwi Agustin Wulandari, Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia