CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT

Authors

  • Ary Andini Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
  • Endah Prayekti Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
  • Devyana Dyah Wulandari Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
  • Ersalina Nidianti Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia

DOI:

https://doi.org/10.33086/ijmlst.v2i1.1467

Keywords:

Collagen, chitosan, BSLT, wound dressing, ultraviolet

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the  best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing  sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into  K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained  collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.

Downloads

Download data is not yet available.

References

Dhivya S, Vijaya V, Santhini E. Review article Wound dressings – a review. BioMedicine. 2015;5(4):24–8.

Lei J, Sun L, Li P, Zhu C, Lin Z, Mackey V, et al. The Wound Dressings and Their Applications in Wound Healing and Management. Heal Sci J. 2019;13:1–8.

Shabunin AS, Yudin VE, Dobrovolskaya IP, Zinovyev E V, Zubov V, Ivan EM, et al. Chitin / Chitosan Nanofibers : Processing and Biomedical Applications. Cosmetics. 2019;6:1–10.

Andini A, Prayekti E. Chitosan as Antifungal in Channa striata Collagen-Chitosan for Wound Healing. Med Heal Sci J. 2019;3(2):8–11.

Leliani HN, Seniwati DS. Collagen Extraction from Bone Of Lutjanus Sp. and Toxicity Assay. Indones Chim Acta. 2019;12(1):67–72.

Mennini N. Quality of wound dressings: A first step in establishing shared Criteria and objective procedures to evaluate their performance. J Wound Care. 2016;25(October 2017).

Kumar PTS, Lakshmanan V, Anilkumar T V, Ramya C, Reshmi P, Unnikrishnan AG, et al. Retraction of “Flexible and Microporous Chitosan Hydrogel/Nano ZnO Composite Bandages for Wound Dressing: In Vitro and In Vivo Evaluation.” ACS Appl Mater Interfaces. 2019;11:28596.

Ariyadi T, Dewi SS. Pengaruh Sinar Ultra Violet terhadap Pertumbuhan Bakteri Bacillus sp. sebagai Bakteri Kontaminan (Effect of Ultra Violet Rays on the Growth of Bacillus sp. as Contaminant Bacteria). J Kesehat. 2009;2:20–5.

B.N Meyer, N.R. Ferrigni, J.E Putnam, L.B Jacobsen, D.E Nichols J. M. Brine Shrimp : A Convenient General Bioassay for Active Plant Constituents. J Med Plant Res. 1982;45:31–4.

Muaja AD, Koleangan HSJ, Runtuwene MRJ. Uji Toksisitas dengan Metode BSLT dan Analisis Kandungan Fitokimia Ekstrak Daun Soyogik ( Saurauia bracteosa DC ) dengan Metode Soxhletasi (Toxicity Assay using BSLT Method and Phytochemical Content Analysis of Soyogik Leaf Extract (Saurauia bracteosa DC) using Soxhletation Method). J MIPA Unsrat Online 2. 2013;2(2):115–8.

Antonio C, Silva DS, Andrade NJ De, Fátima N De, Soares F, Ferreira SO. Evaluation of Ultraviolet Radiation to Control Microorganisms Adhering to Low-Density Polyethylene Films. Brazilian J Microbiol. 2003;34(1517–8382):175–8.

Lei J, Chen P, Li Y, Wang X, Tang S. Collagen hydrogel dressing for wound healing and angiogenesis in diabetic rat models. Int J Clin Exp Med. 2017;10(12):16319–27.

Ou A, Louis H, Pi A, Oc A, Ei N, Ef O. Biochemistry & Pharmacology : Open Access The Chemistry of Chitin and Chitosan Justifying their Nanomedical Utilities. Biochem Pharmacol (Los Angel). 2018;7(1):1–6.

Published

2020-04-09

How to Cite

Andini, A., Prayekti, E., Dyah Wulandari, D. and Nidianti, E. (2020) “CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT”, Indonesian Journal of Medical Laboratory Science and Technology, 2(1), pp. 21-26. doi: 10.33086/ijmlst.v2i1.1467.