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Ally Kafesa Nadira Nur Hajah Lutfi Cep Wahyu

Abstract

The concentration of magnesium is determined based on the absorbance of the Mg-Xylydil Blue-I complex solution use spectrophotometer. Based on the Lambert-Beer rule, the calculation of sample concentration is based on the formula A = Ԑ. b. C. Generally, the thickness of the cuvette (b) and the molar absorptivity (Ԑ) factor will be ignored because it is considered to have a fixed value, therefore the sample concentration is measured based on the ratio of the absorbance of the sample against the standard solution. However, the standard solution contains pure magnesium and has a different matrix than the sample matrix, so this condition can give analytical errors and lead to misinterpretation of the results. The purpose of this study was to determine the accuracy and the precision of serum magnesium calculation by the principle of the Mg- Xylydil Blue-I complex reaction based on molar absorptivity compared to the general method. This research uses comparative study design methods. The serum sample used was the patient's serum specimen who has a normal magnesium level. The results showed that the significance value of the paired t-test statistical was 0.000 (p < 0.05). The accuracy value (d%) of the calculation formula uses Ɛ is 0.00 and the precision value (CV%) is 0.53. While the accuracy value (d%) of the calculation formula without Ɛ is 0.00 and the precision value (CV%) is 0.38. Calculations based on molar absorptivity (Ɛ) can measure more significant serum magnesium than those calculated based on standard magnesium solutions.

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How to Cite
Kafesa, A., Lutfi, N. N. H. and Wahyu, C. (2021) “Chemometric Analysis of Serum Magnesium Calculations Using Mg-Xylidyl Blue-I Method Based on Molar Absorptivity”, Indonesian Journal of Medical Laboratory Science and Technology, 3(1), pp. 9–18. doi: 10.33086/ijmlst.v3i1.1876.
Section
Articles
Epsilon, Magnesium, Mg-Xylydil Blue-I Complex Reaction, Serum, UV-Vis Spectrophotometry

References

Locatelli M, Carlucci G, Genovese S, Curini M, Epifano F. Use of HPLC in the determination of the molar absorptivity of 4′-geranyloxyferulic acid and boropinic acid. Chromatographia. 2011;73:889-896. 10.1007/s10337-011-1979-5.

Schutten JC, Post A, Meer M, Ijmker J, Goorman F, Danel RM, Vervloet MG, Borst MH, Touw DJ, Bakker SJL. Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study. Clin Chim Acta. 2020;510:772-780, https://doi.org/10.1016/j.cca.2020.09.007.

Kimura I, Sekine T, edo Y. Production of calcium magnesium phosphate microspheres in a water-in-oil-in-water dispersion, colloids and surfaces A: physicochemical and engineering aspects. J Colsurfa. 2020;613:126089, https://doi.org/10.1016/j.colsurfa.2020.126089.

Nader R. Guidelines clinical chemistry. Boston Children's Hospital. Boston: Harvard Medical School; 2020.

Coger SAS. 79, rue des Morillons. No. Lot 75015 PARIS Ref. CDX-X0009-G001 CHEMODEX 14936-97-1; 2017

Şenol ZM, Şimşek S, Ulusoy HI, Mahmood A, Kaya S. Insight from adsorption properties of Xylidyl Blue embedded hydrogel for effective removal of uranyl: Experimental and theoretical approaches. Pol Tes. 2020;88:106566, https://doi.org/10.1016/j.polymertesting.2020.106566

Kass L, Rosanoff A, Tanner A, Sullivan K, McAuley W, et al. Effect of transdermal magnesium cream on serum and urinary magnesium levels in humans: A pilot study. PLOS ONE. 2017;12(4): e0174817

Acquah GE, Via BK, Fasina OO, Adhikari S, Billor N, et al. Chemometric modeling of thermogravimetric data for the compositional analysis of forest biomass. PLOS ONE. 2017;12(3): e0172999.

Cooks G, HassHB, Preface. Medical applications of mass spectrometry. Elsevier. 2018:xxi-xxiii, https://doi.org/10.1016/B978-044451980-1.50002-1

Worsfold P, Townshed A, Poole C, Miro M. Encyclopedia of analytical science (Third Edition). Academic Press. 2019:420-424.

PT. DiaSys Diagnostic Systems GmbH. In vitro diagnostic kit magnesium serum. Alte Strasse 9 65558 Holzheim Germany. 2018.

Nugroho B, Pramudya Y, Widodo. The content analysis of Bisphenol A (BPA) on water in plastic glass with varying temperatures and contact times using uv-vis spectrophotometer. Ina Rev Phy. 2019;1(2):27-32.

Junior BRA, Carneiro RL. Chemometrics approaches in forced degradation studies of pharmaceutical drugs. MDPI Molecules. 2019; 24(3804):2-24. http://doi:10.3390/molecules24203804

Rohman A. The employment of Fourier transforms infrared spectroscopy coupled with chemometrics techniques for traceability and authentication of meat and meat products. J Adv Vet Anim Res. 2019;6(1);9-17. http://doi.org/10.5455/javar.2019.f306

Martynko E, Kirsanov Dmitry. Application of chemometrics in biosensing: A brief review. MDPI Biosensors. 2020;10(100): 2-20. http://doi:10.3390/bios10080100

Rohman A, Windarsih A. The application of molecular spectroscopy in combination with chemometrics for halal authentication analysis: A Review. MDPI. Int J Mol Sci. 2019;21(5155):2-18. http://doi:10.3390/ijms21145155

Tsenkova R, Munćan J, Pollner B, Kovacs Z. Essentials of aquaphotomics and its chemometrics approaches. Front. Chem. 2018;6:363 http://doi: 10.3389/fchem.2018.00363

Pieters M, Guthold M, Nunes CM, Lange Z. Interpretation and validation of maximum absorbance data obtained from turbidimetry analysis of plasma clots. Departement of Health and human service-USA. 2020;120(1):44–54. http://doi:10.1055/s-0039-1698460.

Workinger JL, Doyle RO, Bortz J. Challenges in the diagnosis of magnesium status. MDPI Nutrients. 2018;10(9);1202. http://doi:10.3390/nu10091202

Rompas GR, Kaligis SHM, Tiho M. Comparasion of magnesium levels before and after heavy-intensity physical activity. Jurnal e-Biomedik (eBm). 2015; 3(2).

Ally Kafesa, Medical Laboratory of Technology Study Program, Rajawali Institute of Health, Bandung, Indonesia

Nadira Nur Hajah Lutfi, Medical Laboratory of Technology Study Program, Rajawali Institute of Health, Bandung, Indonesia

Cep Wahyu, Medical Laboratory of Technology Study Program, Rajawali Institute of Health, Bandung, Indonesia