Phytochemical and Toxicity Analysis of Leucas zeylanica crude extracts
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Abstract
Leucas zeylanica (L.) R. Br. (L. zeylanica) originated from Lamiaceae family which is also known as “Pokok ketumbit” by local Malaysian is famous for therapeutic treatment uses especially in India and Sri Lanka. Throughout this study, four different solvents which were n-hexane, chloroform, methanol, and distilled water used in the extraction method using cold maceration technique. Optimization was done and methanol became the best solvent that produced highest percentage yield compared to the other solvents and also been chose to carry out a few other analysis. The extracts were subjected to qualitative and quantitative analyses to determine the phytochemical constituents present in aerial parts of L. zeylanica. The Fourier Transform Infrared spectroscopy – Attenuated Total Reflection (FTIR-ATR) results showed few significance peaks according to extracted solvents. Qualitative analysis on the methanolic extract showed that L. zeylanica contains phenol, flavonoid and tannin through phytochemical screening tests using colorimetric method. The Gas Chromatography – Mass Spectrometry (GC-MS) results demonstrated few fatty acids been extracted in both n-hexane and chloroform extracts with high peak area, while Liquid Chromatography Tandem with Mass Spectrometry (LC-MS-MS) results identified that chloroform extract showed fragment spectrum of tricin [M+H]+ ion at m/z 328.1 with retention time of 19.49 min, while methanol extract had two fragment spectrum of tricin and apigenin at m/z 282.3 and 270.4 in positive ion mode at 19.53 min and 16.70 min respectively. Quantitative analysis on methanolic extract was done via Ultra Violet-visible spectrophotometric assay for estimation of total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC). TPC showed a gallic acid standard calibration curve, y = 0.0063x + 0.02 and estimated amount was 151.54 ± 0.04 mg of gallic acid equivalent/ 1 g of extract, meanwhile TFC displayed a quercetin standard calibration curve of y = 0.0050x + 0.037 and estimated amount of flavonoids was 71.76 ± 0.2 mg of quercetin equivalent/ 1 g of extract. Additionally, The toxicity test for the L. zeylanica extract showing that this compound is safe and non-toxic in term of skin irritation, cytotoxicity and also genotoxicity.
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Copyright (c) 1970 Faizuan Abdullah
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References
Abdullah, F., Nasir, S., Han, D. K., Appalasamy, S., Nor, M. M., & Rak, A. E. (2019). Potential of Leucas zeylanica extract to eliminate E. coli and S. aureus in Corbicula fluminea ("Etak") tissue. Malaysian Journal of Fundamental and Applied Sciences, 15(4), 597-599. Retrieved from
Choudhary, A. K., Sunojkumar, P., & Mishra, G. (2017). Fatty acid profiling and multivariate analysis in the genus Leucas reveals its nutritional, pharmaceutical and chemotaxonomic significance. Phytochemistry, 143, 72-80. doi:https://doi.org/10.1016/j.phytochem.2017.07.007
De Jong, W. H., Carraway, J. W., & Geertsma, R. E. (2020). 6 - In vivo and in vitro testing for the biological safety evaluation of biomaterials and medical devices. In J. P. Boutrand (Ed.), Biocompatibility and Performance of Medical Devices (Second Edition) (pp. 123-166): Woodhead Publishing.
Ferraz, D., Thomaz, D. V., Antunes, R. S., & Lopes, F. M. (2021). Development of a low-cost colorimetric paper-based spot test for the environmental monitoring of phenolic pollutants. Environmental Challenges, 100128. doi:https://doi.org/10.1016/j.envc.2021.100128
Hung, N. H., Chuong, N. T. H., Satyal, P., Hieu, H. V., Dai, D. N., Huong, L. T., Setzer, W. N. (2019). Mosquito larvicidal activities and chemical compositions of the essential oils of Leucas zeylanica growing wild in Vietnam. Natural Product Communications, 14(6), 1-7. doi:10.1177/1934578X19842675
Kiranda, H. K., Mahmud, R., Abubakar, D., & Zakaria, Z. A. (2018). Fabrication, Characterization and Cytotoxicity of Spherical-Shaped Conjugated Gold-Cockle Shell Derived Calcium Carbonate Nanoparticles for Biomedical Applications. Nanoscale Research Letters, 13(1), 1. doi:10.1186/s11671-017-2411-3
Mohd Bakhori, S. K., Mahmud, S., Mohamad, D., Masudi, S. a. M., & Seeni, A. (2021). Cytotoxicity determination of nano-zinc oxide eugenol on human gingival fibroblast cells. Materials Chemistry and Physics, 124649. doi:https://doi.org/10.1016/j.matchemphys.2021.124649
Napagoda, M., Gerstmeier, J., Butschek, H., Lorenz, S., Kanatiwela, D., Qader, M., Werz, O. (2018). Lipophilic extracts of Leucas zeylanica, a multi-purpose medicinal plant in the tropics, inhibit key enzymes involved in inflammation and gout. Journal of Ethnopharmacology, 224, 474-481. doi:https://doi.org/10.1016/j.jep.2018.04.042
Nidhal, N., Zhou, X.-M., Chen, G., Zhang, B., Han, C., & Song, X. (2020). Chemical constituents of Leucas zeylanica and their chemotaxonomic significance. Biochemical Systematics and Ecology, 89, 104006. doi:https://doi.org/10.1016/j.bse.2020.104006
Priya, R., Nirmala, M., Shankar, T., & Malarvizhi, A. (2018). Phytochemical Compounds of Leucas aspera L. In.
Saritha, K., Rajesh, A., Manjulatha, K., Setty, O. H., & Yenugu, S. (2015). Mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L., and Tridax procumbens (L.) R. Br. ex Schult. Frontiers in Microbiology, 6(577). doi:10.3389/fmicb.2015.00577
Schaumlöffel, L. S., Fontoura, L. A. M., Santos, S. J., Pontes, L. F., & Gutterres, M. (2021). Vegetable tannins-based additive as antioxidant for biodiesel. Fuel, 292, 120198. doi:https://doi.org/10.1016/j.fuel.2021.120198
Vijayarathna, S., & Sasidharan, S. (2012). Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pacific Journal of Tropical Biomedicine, 2(10), 826-829. doi:https://doi.org/10.1016/S2221-1691(12)60237-8
