Zamharirah Bt Ahamad Mashat Faizuan Abdullah Asnida Abdul Wahab Muhammad Faiz Md Shakhih Anis Suzziani Roslan


Enzyme-based sensors frequently produce unsatisfactory results such as poor reproducibility and insufficient long-term stability due to the natural instability of enzymes, stringent experimental conditions, and complicated immobilization procedures. Thus, an electrochemical non enzymatic sensor was fabricated by deposition of the multi-walled carbon nanotube (MWCNT) with zinc oxide nanoparticles (ZnO NP) and also molecular imprinted polymer (MIP) on a screen-printed carbon electrode (SPCE). Then, the modified electrode (SPCE/MWCNT/ZnO/MIP) was formed on the surface area of the SPCE. This study wanted to demonstrate the glucose detection between molecular imprinted polymer (MIP) which contained glucose as template, o-phenylenediamine (oPD) and potassium persulfate as initiators in 0.1 M PBS at pH 7 and   non-imprinted   polymer (NIP) without   addition   of   the   template.   The characterization and evaluation of various factor such as sensitivity, selectivity and limit of detection (LOD) were investigated through cyclic voltammetry (CV) and scanning electron microscopy (SEM) was used to look up onto the surface area of the modified electrode. The SPCE/MWCNT/ZnO/MIP electrode sensor showed a linear glucose concentration range from 0, 0.5, 1, 2 to 5 mM (R2 = 0.9709). The sensitivity of the sensor was 0.3386 μA mM-1 cm-2 with low detection limit of 1.81 mM. The sensor showed good stability and reproducibility along with excellent anti-interference properties to ascorbic acid, lactic acid, tartaric acid, and acetic acid. Finally, the applicability of the as-prepared SPCE/MWCNT/ZnO/MIP electrode sensor was successfully studied for detection of glucose. The results obtained for our sensor confirm that it is a promising non-enzymatic glucose sensor to be used for practical purpose.


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How to Cite
Bt Ahamad Mashat, Z. ., Abdullah, F. ., Abdul Wahab, A. ., Faiz Md Shakhih, M. ., & Suzziani Roslan, A. . (2022). Development of non-enzymatic screen-printed carbon electrode sensor for glucose using cyclic voltammetry. Environmental and Toxicology Management, 2(1), 14–20. https://doi.org/10.33086/etm.v2i1.2542
Non-enzymatic sensor, screen printed carbon electrode, molecular imprinted polymer (MIP), modified electrode (SPCE/MWCNT/ZnO/MIP), cyclic voltammetry


Babu, T. G. S. and Ramachandran, T., 2010. Development of highly sensitive non-enzymatic sensor for the selective determination of glucose and fabrication of a working model. Electrochimica Acta. 55, 1612-1618

Baomin, W. and Bo, P., 2020. The Influence of N,N-Dimethylformamide on Dispersion of Multi-Walled Carbon Nanotubes. Russian Journal of Physical Chemistry A. 94, 810-817

Chaiyo, S., Mehmeti, E., Siangproh, W., Hoang, T. L., Nguyen, H. P., Chailapakul, O. and Kalcher, K., 2018. Non-enzymatic electrochemical detection of glucose with a disposable paper-based sensor using a cobalt phthalocyanine–ionic liquid–graphene composite. Biosensors and Bioelectronics. 102, 113-120

Diouf, A., Bouchikhi, B. and El Bari, N., 2019. A nonenzymatic electrochemical glucose sensor based on molecularly imprinted polymer and its application in measuring saliva glucose. Materials Science and Engineering: C. 98, 1196-1209

Dong, Q., Ryu, H. and Lei, Y., 2021. Metal oxide based non-enzymatic electrochemical sensors for glucose detection. Electrochimica Acta. 370, 137744

Ibrahim, A. A., Umar, A., Ahmad, R., Kumar, R. and Baskoutas, S., 2016. Fabrication and Characterization of Highly Sensitive and Selective Glucose Biosensor Based on ZnO Decorated Carbon Nanotubes. Nanoscience and Nanotechnology Letters. 8, 853-858

Leitzen, S., Vogel, M., Engels, A., Zapf, T. and Brandl, M., 2021. Identification and quantification of glucose degradation products in heat-sterilized glucose solutions for parenteral use by thin-layer chromatography. PLOS ONE. 16, e0253811

Leong, K. L., Ho, M. Y., Lee, X. Y. and Yee, M. S.-L., 2020. A Review on the Development of Non-Enzymatic Glucose Sensor Based on Graphene-Based Nanocomposites. Nano. 15, 2030004

Ndiaye, A. L., Delile, S., Brunet, J., Varenne, C. and Pauly, A., 2016. Electrochemical Sensors Based on Screen-Printed Electrodes: The Use of Phthalocyanine Derivatives for Application in VFA Detection. Biosensors. 6, 46

Onor, M., Gufoni, S., Lomonaco, T., Ghimenti, S., Salvo, P., Sorrentino, F. and Bramanti, E., 2017. Potentiometric sensor for non invasive lactate determination in human sweat. Analytica Chimica Acta. 989, 80-87

Rezaei, B., Rahmanian, O. and Ensafi, A. A., 2014. An electrochemical sensor based on multiwall carbon nanotubes and molecular imprinting strategy for warfarin recognition and determination. Sensors and Actuators B: Chemical. 196, 539-545

Sridara, T., Upan, J., Saianand, G., Tuantranont, A., Karuwan, C. and Jakmunee, J., 2020. Non-Enzymatic Amperometric Glucose Sensor Based on Carbon Nanodots and Copper Oxide Nanocomposites Electrode. Sensors. 20, 808

Wei, H., Xue, Q., Li, A., Wan, T., Huang, Y., Cui, D., Pan, D., Dong, B., Wei, R., Naik, N. and Guo, Z., 2021. Dendritic core-shell copper-nickel alloy@metal oxide for efficient non-enzymatic glucose detection. Sensors and Actuators B: Chemical. 337, 129687

Wu, H., Tian, Q., Zheng, W., Jiang, Y., Xu, J., Li, X., Zhang, W. and Qiu, F., 2019. Non-enzymatic glucose sensor based on molecularly imprinted polymer: a theoretical, strategy fabrication and application. Journal of Solid State Electrochemistry. 23, 1379-1388

Zamharirah Bt Ahamad Mashat, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia

Faizuan Abdullah, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia

Asnida Abdul Wahab, School of Biosciences andMedical Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia

Muhammad Faiz Md Shakhih, School of Biosciences andMedical Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia

Anis Suzziani Roslan, School of Biosciences and Medical Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia