Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD

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Published Apr 26, 2023
DOI https://doi.org/10.33086/mtphj.v7i1.3837

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chylen setiyo rini
universitas muhammadiyah sidoarjo

Abstract

Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease that attacks the intestines. This study aims to determine the kaffir lime leaf extract in inhibiting the growth of Bacillus cereus bacteria using the Time-Kill Test method. This study used the Minimum Inhibitory Concentration (MIC) and Time-Kill Test methods. The MIC results in kaffir lime leaf extract that can inhibit bacteria at 2 mg/mL, while the Time-Kill Test shows a death phase at 24 hours. Kaffir lime leaf extract has a bacteriostatic effect at 0, 4, 8, and 24 hours, while amoxicillin and the combination of amoxicillin and extract have a bacteriostatic effect at 4, 8, and 24 hours. Based on the results of the One Way ANOVA test with a 95% confidence level (p <0,05) showed a significant effect of kaffir lime leaf extract on the growth of Bacillus cereus.

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How to Cite
setiyo rini, chylen. (2023). Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD. Medical Technology and Public Health Journal, 7(1), 61–66. https://doi.org/10.33086/mtphj.v7i1.3837
Issue
Vol. 7 No. 1 (2023): Medical Technology and Public Health Journal March 2023
Section
Articles

Copyright (c) 2023 chylen setiyo rini

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Bacillus cereus, Kaffir Lime Leaves (citrus hystrix), MIC, time kill tets

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