The Effect of Sembukan Leaf Extract (Paederia Foetida) on the Growth of Klebsiella Pneumoniae Bacteria with the Disc Method and the Contact Method


chylen setiyo rini
Nova Linda Pratiwi


Sembukan leaf (Paederia foetida) is a wild plant which is known to have many benefits and can be used as a medicinal plant. These plants contain bioactive compounds that function as antibacterial. This study aims to determine the ability of sembukan leaf extract to inhibit the growth of Klebsiella pneumoniae. The methods used in this study were disc diffusion and contact methods, with variations in the concentration of curd leaf extract 0%, 25%, 50%, 75% and 100%. The research data obtained were analyzed by One Way ANOVA with 95% confidence level (p<0.05). The best concentration with the disc diffusion method at a concentration of 75% with an inhibitory diameter of 15.25 mm with a strong category and the best percentage of inhibition method on sembukan leaf extract at 69.42% with a bacteristatic category at a concentration of 75%, while the results of the One Way ANOVA test that has been done obtained a significant value of p=0.000 (p<0.05) indicates a significant effect on the administration of sembukan leaf extract against Klebsiella pneumoniae bacteria. The results showed that sembukan leaf extract was able to inhibit the growth of Klebsiella pneumoniae.


How to Cite
setiyo rini, chylen, & Pratiwi, N. L. (2022). The Effect of Sembukan Leaf Extract (Paederia Foetida) on the Growth of Klebsiella Pneumoniae Bacteria with the Disc Method and the Contact Method. Medical Technology and Public Health Journal, 6(2), 185–194.


  1. Abriyanto, Elvian, A., & Sabikis, S. (2012). ANTI-FUNCIAL ACTIVITY OF SEMBUKAN LEAF ETHANOL EXTRACT (Paederia foetida L) AGAINST Candida albicans. Pharmacy, 09(03), 1–10.
  2. Agfadila, T., Sandhi, PA, & Puspawati, NN (2017). Inhibitory Ability of Pegagan Leaf Extract (Centella asiatica (L.) Urban) Against the Growth of Escherichia coli ATCC 8739. Journal of ITEPA, 6(2), 21–29.
  3. Badrunasar, A. and HSB (2017). Medicinal Efficacious Wild Plants. In Book of Wild Plants with Medicinal Efficacy: Vol. ISBN 978-6.
  4. Brooks GF, Carroll KC, Butel JS, Morse SA, Mietzner TA. (2013). Jawetz, Melnick, & Adelberg's medical microbiology. 26th Edition. EGC Medical Book Publisher Translation, 2010. Medical microbiology Jawetz, Melnick, & Adelberg. Edition 25. Jakarta: EGC Medical Publisher
  5. Chauhan, K., Patel, A., Patel, M., Macwan, C., Solanki, R., & Adeshara, S. (2010). Paederia foetida Linn. As a potential medicinal plant : A Review. Journal of Pharmacy Research, 3(12), 3135–3137.
  6. CLSI (Clinical and Laboratory Standards Institute). (2018). Performance Standards For Antimicrobial Susceptibility Testing. USA: Twenty-Sevenft Informational Supplement.
  7. Dewi, ZI, Susetyarini, RE, & Latifa, R. (2020). Effect of Leaf Extract (Paederia foetida) on the Inhibitory Zone of Escherichia coli (Used as a source of learning biology). 266–271. Retrieved from
  8. Hidayat, A., Selamet Duniaji, A., & Indri Hapsari Arihantana, NM (2020). RESISTANCE TEST OF LEAVE EXTRACT (Paederia foetida) AGAINST Vibrio cholerae. Journal of Food Science and Technology (ITEPA), 9(4), 390. DOI:
  9. Himawan, DSA (2021) Inhibitory Test of Fresh Bitter Fruit Extract (Momordica charantia Liin) Against Bacteria Klebsiella pneumoiniae and Streptococcus pneumoniae) (Undergraduated Thesis). Faculty of Health Sciences, University of Muhammadiyah Sidoarjo
  10. Huda, C., Amalia Eka P., Devri Windri S. (2019) Test of Antibacterial Activity of the Fraction of Maserat Zibethinus Folium Against Escherichia Coli. Journal of ScienceHealth. 3(1), pp. 7-14. DOI:
  11. Ismawati, Destryana, RA, & Hodri, S. (2020). UTILIZATION OF KASEMBUKAN (Paedria foetida Linn.) AS A TRADITIONAL MEDICINE FOR SUMENEP COMMUNITY. Proceedings of the National Seminar on Research Results and Community Service with the Theme “Modern and Traditional Health” UTILIZATION, 208.
  12. Kusuma, DA (2013) Differences in Sensitivity Patterns to AntibioticsKlebsiella Sp. Who Colonize Toddler Nasopharyngeal (Undergraduate's thesis). UNDIP, Faculty of Medicine, Semarang. Retrieved from
  13. Kuswiyanto, (2016) Bacteriology Health Analyst Textbook 1. Jakarta:EGC
  14. Maleki, S., Seyyednejad, SM, Mirzaie Damabi, N., & Motamedi, H. (2008). Antibacterial activity of the fruits of Iranian Torilis leptophylla against some clinical pathogens. Pakistan Journal of Biological Sciences, 11(9), 1286–1289. DOI:
  15. Mardjono, M. (2012). Pharmacology and Therapy Edition 5. Jakarta: FKUI Publishing Agency
  16. May, D. (2016).The Effect of Beluntas Leaf Infusion (Pluchea Indica) on the Growth of Staphylococcus Epidermidis Bacteria. Unnes Journal of Life Science. 4(1), 60–65. Retrieved from
  17. Ningsih, D., Zusfahair., & Kartika, D. (2017). Activity of Soursop Leaf Chloroform Extract (Annona Muricata L) As Antibacterial Against Propionibacterium Acnes. Scientific Research Journal, 22(2), 93.
  18. Nurhayati, LS, Yahdiyani, N., & Hidayatulloh, A. (2020). Comparison of Testing the Antibacterial Activity of Yogurt Starter with the Well Diffusion Method and the Disc Diffusion Method. Journal of Animal Husbandry Technology, 1(2):41-46. DOI:
  19. Osman, H., Rahim, AA, Isa, NM, & Bakhir, NM (2009). Antioxidant activity and phenolic content ofPaederia foetida and Syzygium aqueum. Molecules, 14(3), 970–978. DOI:
  20. Fasting, NS, Fatimawali, F., & Wiyono, W. (2019). Antibacterial Activity Test of Red Galangal (Alpinia Purpurata K. Schum) Rhizome Extract Against Klebsiella Pneumonia Bacteria Isolate Urine in Patients with Urinary Tract Infections. Pharmacon, 8(4), 982. DOI:
  21. Ramadhan, MR, Pratiwi, IDPK, & Hapsari A., NMI (2020). Inhibitory POWER TEST OF FRUIT EXTRACT (Ficus racemosa Linn) ON THE GROWTH OF Staphylococcus aureus ATCC 25923. Journal of Food Science and Technology (ITEPA), 9(1), 38. i01.p05 DOI:
  22. Rose Simanungkalit, E., Selamet Duniaji, A., & Ekawati, IGA (2020). Flavonoid Content and Antibacterial Activity Ethanol Extract of Sintrong Leaves (Crassocephalum crepidiodes) Against Bacillus cereus Bacteria. Journal of Food Science and Technology (ITEPA), 9(2), 202. DOI:
  23. Schroll, C., Barken, KB, Krogfelt, KA, & Struve, C. (2010). Role of type 1 and type 3 fimbriae in Klebsiella pneumoniae biofilm formation. BMC Microbiology, 10. DOI:
  24. Utomo, SB, Fujiyanti, M., Lestari, WP, & Mulyani, S. (2018). Antibacterial Activity Test of Hexadecyltrimethylammonium-Bromide Compounds Against Staphylococcus Aureus AND Escherichia Coli 3(3), 201–209. DOI:
  25. Vitalia, PA, (2021). The Effect of Giving Extract of Coarse Leaf Extract (Paederia foetida L) Against Inhibition Of Pseudomonas Aeruginosa Bacteria In Vitro (Undergraduate's thesis). Faculty of Fisheries and Marine Science Universitas Brawijaya: Malang