Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C
Article Sidebar
Main Article Content
Abstract
Pneumonia is an infection that causes the largest death in children worldwide (South Sumatra Province), which occupies the 6th position. One of the laboratory re-identification of Klebsiella pneumonia is a multi-antibiotic testing. The high sensitivity antibiotic that is still used is amikacin. The uniqueness of the diameter of the inhibition zone using the antibiotic amikacin is that it is active against most gram-negative bacilli. But one of the several factors that affect the diameter of the inhibition zone is the incubation temperature. The optimum temperature for pathogenic bacteria is 37oC by using an incubator; however, several factors in the use of instruments such as frequent instability and disruption of installation lead to a need of incubation at 25oC. The study aimed to determine the difference in the diameter of the growth inhibition zone of K. pneumoniae after incubation at 37oC and 25oC. This research is an experimental research conducted at the Microbiology Laboratory of IKesT Muhammadiyah Palembang. The sample is K. pneumoniae which will be subjected to gram staining, biochemical tests, followed by a sensitivity test on Mueller Hinton media which is given an amikacin antibiotic disk and incubated at 370C and 250C in order to calculate the diameter of the zone of inhibition for the growth of K. pneumoniae bacteria. The data was analyzed using the alternative Wilcoxon test which obtained a p value of 0.014. The results of this investigation showed that K. pneumoniae incubated at 37°C and 25°C had a significantly different diameter of the growth inhibition zone.
Downloads
Article Details
Copyright (c) 2022 Juwy Trianes, Bastian Bastian, Dewi Hartati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Sari DP, Rahmawati R, Elvi RPW. Detection and identification of coliform bacteria genera isolated from aloe drink. J Labora Med. 2019;3(1):29–35. https://doi.org/10.26714/jlabmed.3.1.2019.29-35
Provincial Health Office. Deconcentralized annual performance plan of the health office; South Sumatra Province Government; 2018. 1976100719960
Lasut MRC, Fatimawali F, Antasionasti I. Resistance test of nanoparticles extract of rhizome of red galangal (Alpinia purpurata k. schum) on the growth of Klebsiella Pneumoniae bacteria in urine islates in patients with resistant antibiotic flash infections. Pharmacon. 2019;8(4):870. https://doi.org/10.35799/pha.8.2019.29364 DOI: https://doi.org/10.35799/pha.8.2019.29364
Azizah R, Antarti AN. Antibacterial activity test of the extract and sap of the banana leaf kepok kuning (Musa paradisiaca linn.) against Pseudomonas aeruginosa aAnd Klebsiella pneumoniae bacteria using the agar diffusion method. Jpscr J Pharm Sci Clin Res. 2019;4(1):29. 10. DOI.: 20961/jpscr.v4i1.26544 DOI: https://doi.org/10.20961/jpscr.v4i1.26544
Kambuno DNTF. Identification of gram-negative bacteria extended spectrum beta lactamase in the nicu room at RSUD Prof. Dr. W. Z. Johannes Kupang. J Health Info [Internet]. 2017;15(2):333–45. https://orcid.org/0000-0002-5085-3584. DOI: 10.31.1965.
Baron S, Larvor E, Jouy E, Kempf I, Le Bouquin S, Chauvin C, Et Al. Agreement between the categorization of isolates of Aeromonas salmonicida and Yersinia ruckeri by disc diffusion and mic tests performed at 22℃. J Fish Dis. 2021;44(7):979–85. https://doi.org/10.1111/jfd.13356 DOI: https://doi.org/10.1111/jfd.13356
Taswin M, Astuti RD, Handayani BT. Clavulanate In Dry Syrup Preparation Against The effect of storage temperature of the combination of amoxicillin and clavulanic acid in dry syrup preparations on inhibition of stockhouseaure bacteria poltekk. 2021;15(1):40–9. 10. DOI: 36086/jpp.v16i1.690.
Novard MFA, Suharti N, Rasyid R. Overview of bacteria that cause infection in children by specimen type and pattern of resistance in the laboratory of Dr. RSUP. M. Djamil Padang 2014-2016. Andalas Health Journal, 2019, 8(2S), p. 26. Available at: https://doi.org/10.25077/jka.v8i2s.955. DOI: https://doi.org/10.25077/jka.v8i2S.955
Zeniusa P, Ramadhian MR, Nasution SH, Karima N. In vitro inhibitory test of green tea ethanol extract against Escherichia coli. Majority. 2019;8(2):136–43. ISSN: 2337-3776. DOI: 10.23960/jkunila13525-529
Hasan TH, Shlash SA, Jasim SA, Hussein EF, Alasedi KK, Aljanaby AA. The epidemiology of Klebsiella pneumoniae: A review. Ann Rom Soc Cell Biol. 2021;25 (4):8848–60. ISSN: 20678282 https://doi.org/10.1016/0277-9536(95)00112
Saïd1 A, Benacer S, Daoudi Y, Kraimat M, Hadj A. Effect of temperature on the antibiotic sensitivity test of Escherichia coli HO25 by agar diffusion method. J Adv Res in Sci and Technol. 2018; 5(1), 640-647. ISSN: 2352-9989 DOI: 10.48175/568 DOI: https://doi.org/10.48175/568
Karwiti W, Basa IH, Asrori A, Silvia V. Gambaran kadar iodium (sebagai KIO3) dalam garam dapur yang di jual di pasar kota palembang tahun 2017 DESCRIPTION OF IODIUM LEVELS (As KIO3) in kitchen salt sold at the market of city of Palembang in 2017. Palembang Poltekkes Health Journal) 2018;13(2):75–9. https://doi.org/10.36086/jpp.v13i2 DOI: https://doi.org/10.36086/jpp.v13i2.233
Suarjana IGK, Besung INK, Mahatmi H, Tono K. Isolation and identification of bacteria. Udayana University Faculty of Veterinary Medicine Module. 2017;1–29. https://simdos.unud.ac.id/uploads/file_pendidikan_1_dir/72be8d6f4c3edc1ec4fb976960f3a7b5.pdf
Nuryadi, Astuti TD, Utami ES, Budiantara M. Textbooks on the basics of research statistics. 2017. ISBN: 978-602-6558-04-6.
Faridah DN, Erawan D, Sutriah K, Hadi A, Budiantari F. Implementation of SNI ISO/IEC 17025:2017 - General requirements for competence of testing laboratories and calibration laboratories. National Standardization Body. 2018; 10. DOI: 20473/jkl.v12i2.2020.97-106
Rudianto. The Effect of e-learning relationships in mafiki courses at the sumatran institute of technology using the wilcoxon method. Indonesian Journal of Applied Mathematics. 2020; 1(1), pp. 1–5. https://doi.org/10.35472/indojam.v2i1 DOI: https://doi.org/10.35472/indojam.v2i1
Smith P, Finnegan W, Ngo T, Kronvall G. Influence of incubation temperature and time on the precision of mic and disc diffusion antimicrobial susceptibility test data. Aquaculture. 2018;490: 19–24. 10. DOI: 1016/j.aquaculture.2018.02.020 DOI: https://doi.org/10.1016/j.aquaculture.2018.02.020
Rahman IW, Prihartini A. Test of antibiotic sensitivity to growth of Klebseilla pneumonia from sputum of patients with lower respiratory tract infections. 2021, 3, pp. 81–87. https://doi.org/10.36339/jhest.v3i2.53 DOI: https://doi.org/10.36339/jhest.v3i2.53
Cahyati WH, Sari MP. Trends in toddler pneumonia in Semarang City 2012-2018. Higeia J Public Heal. 2019;3(3):408. https://doi.org/10.15294 /higeia/v3i3/30266
Halla S, Rohmi R. Agrijanti, A. The effectiveness of portable incubators as an innovation tool to support microbiology laboratories. JAMBS. 2019; 6(1): 66. https://doi.org/10.32807/jambs.v6i1.126. DOI: https://doi.org/10.32807/jambs.v6i1.126
