Main Article Content

Durrotul Hasanah Nour Athiroh Abdoes Sjakoer Nurul Jadid Mubarakati

Abstract

Tea and mango mistletoes have to lower blood pressure by improving oxidative stress and endothelial dysfunction. The flavonoid compounds found in these two mistletoes act as antihypertensives. Hypertension is a risk factors that influences the incidence of cardiac disease. This study examines the histopathological of the cardiac of hypertensive rats (preventive model) after treatment of methanolic extract combination of tea and mango mistletoes. This study used a completely randomized experimental design with 25 Wistar rats in total for five treatments, namely negative control (-), positive control (+), treatment 1 (dose of 50 mg/kg BW), treatment 2 (doses of 100 mg/kg BW), and treatment 3 (doses of 200 mg/kg BW). In this study, Deoxycorticosterone acetate (DOCA) were used to induce hyperttension in experimental animals. Data were analysed with one-way ANOVA test using JAMOVI (version 1.1.9.0) and posteriori. There was a significant difference between all groups (p<0.05). Administration of a methanolic extract combination of tea and mango mistletoes as the preventive model can significantly reduce hypertrophy of cardiac muscle cells. This effect occured with a dose of 50 mg/kg BW, the optimal dose to reduce hypertrophy of cardiac muscle cells in Wistar rats male.

Downloads

Download data is not yet available.

Article Details

How to Cite
Hasanah, D., Sjakoer, N. A. A. and Mubarakati, N. J. (2022) “Histopathological Profile of Therapeutic Doses of Methanolic Extract Combination of Tea and Mango Mistletoes in Cardiac of Preventive Hypertensive Rats (DOCA-salt) Model”, Indonesian Journal of Medical Laboratory Science and Technology, 4(2), pp. 99–110. doi: 10.33086/ijmlst.v4i2.2693.
Section
Articles
Hypertension, Hypertrophy, Muscle Cells Cardiac, Mango mistletoe, Tea Mistletoe

References

Annisatul H, Athiroh N, Mubarakati NJ. Histopathological profile of therapeutic doses of mango mistletoe methanolic. JSMARTech. 2021;02(02):48–54. DOI: https://doi.org/10.21776/ub.jsmartech.2021.002.02.48 DOI: https://doi.org/10.21776/ub.jsmartech.2021.002.02.48

Athiroh N, Purnomo Y, Mubarakati NJ. Sub chronic diagnosis of administration with Scurrula atropurpurea to blood biochemichal analysis. IOP Conf Ser Mater Sci Eng. 2020;846(1). DOI: 10.1088/1757-899X/846/1/012002 DOI: https://doi.org/10.1088/1757-899X/846/1/012002

Kurniawan A, Yanni M. Pemeriksaan fungsi endotel pada penyakit kardiovaskular. [Examination of endothelial function in cardiovascular disease]. Hum Care J. 2020;5(3):638. DOI: http://dx.doi.org/10.32883/hcj.v5i3.817 DOI: https://doi.org/10.32883/hcj.v5i3.817

Ayun AQ, Faridah DN, Yuliana ND, Andriyanto A. Pengujian toksisitas akut LD50 infusa benalu teh (Scurrula sp.) dengan menggunakan mencit (Mus musculus). [Testing the acute toxicity of LD50 infusion of parasitic tea (Scurrula sp.) using mice (Mus musculus)]. Acta Vet Indones. 2021;9(1):53–63. DOI: https://doi.org/10.29244/avi.9.1.53-63 DOI: https://doi.org/10.29244/avi.9.1.53-63

Athiroh N, Permatasari N, Sargowo D, Widodo MA. Antioxidative and blood pressure-lowering effects of Scurrula atropurpurea on deoxycorticosterone acetate-salt hypertensive rats. Biomarkers Genomic Med. 2014;6(1):32–6. Available from: http://dx.doi.org/10.1016/j.bgm.2014.01.001 DOI: https://doi.org/10.1016/j.bgm.2014.01.001

Athiroh N, Permatasari N, Sargowo D, Widodo MA. Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats. Iran J Basic Med Sci. 2014;17(8):622–5.

Manosroi W, Williams GH. Genetics of human primary hypertension: focus on hormonal mechanisms. Vol. 40, Endocrine Reviews. 2018. 825–856 p. DOI: https://doi.org/10.1210/er.2018-00071 DOI: https://doi.org/10.1210/er.2018-00071

Syntya A, Masyarakat FK, Surabaya K. Hypertension and heart disease: literature review. 2021;11:541–50. DOI: https://doi.org/10.32583/pskm.v11i4.1621

Lousiana M, Irramah M. Myocardial stunning. Maj Kedokt Andalas. 2017;40(2):100. DOI: http://dx.doi.org/10.22338/mka.v40.i2.p100-110.2017 DOI: https://doi.org/10.22338/mka.v40.i2.p100-110.2017

Rossier BC, Bochud M, Devuyst O. The hypertension pandemic: An evolutionary perspective. Physiology. 2017;32(2):112–25. DOI: https://doi.org/10.1152/physiol.00026.2016 DOI: https://doi.org/10.1152/physiol.00026.2016

Kawabe H, Azegami T, Takeda A, Kanda T, Saito I, Saruta T, et al. Features of and preventive measures against hypertension in the young. Hypertens Res [Internet]. 2019;42(7):935–48. DOI: http://dx.doi.org/10.1038/s41440-019-0229-3. DOI: https://doi.org/10.1038/s41440-019-0229-3

Ayu A, Adi M. Perubahan histopatologi otot jantung dan aorta mencit jantan pascapaparan asap rokok elektrik. [Histopathological changes of heart muscle and aorta in male mice after exposure to e-cigarette smoke]. 2019;8(4):541–51. DOI: 10.19087/imv.2019.8.4.541

Ediriweera DS, Dilina N, Perera U, Flores F, Samita S. Risk of low birth weight on adulthood hypertension - Evidence from a tertiary care hospital in a South Asian country, Sri Lanka: A retrospective cohort study. BMC Public Health. 2017;17(1):15–20. DOI: 10.1186/s12889-017-4268-x DOI: https://doi.org/10.1186/s12889-017-4268-x

Chairunnisa S, Wartini NM, Suhendra L. Pengaruh Suhu dan waktu maserasi terhadap karakteristik ekstrak daun bidara (Ziziphus mauritiana L.) sebagai sumber saponin. [Effect of temperature and maceration time on the characteristics of bidara leaf extract (Ziziphus mauritiana L.) as a source of saponins]. J Rekayasa dan Manaj Agroindustri. 2019;7(4):551. DOI: https://doi.org/10.24843/JRMA.2019.v07.i04.p07 DOI: https://doi.org/10.24843/JRMA.2019.v07.i04.p07

Pratama APC, Berata IK, Samsuri S, Merdana IM. Pengaruh pemberian vitamin E dan deksametason terhadap gambaran histopatologi jantung tikus putih jantan. [Effect of administration of vitamin E and dexamethasone on the histopathological picture of male white rats heart}. Bul Vet Udayana. 2018;10(2):147. DOI: 10.24843/bulvet.2018.v10.i02.p07 DOI: https://doi.org/10.24843/bulvet.2018.v10.i02.p07

Lestari TA, Athiroh N, Mubarakati NJ. Toxicity test of methanolic extract combination of benalu tea leaves and benalu mango leaves against lipid profile of female rats (Rattus norvegicus) on 28-day sub-chronic exposure. Known Nat. 2020;3 (1):22–8. DOI:10.33474/j.sa.v3i1.6621 DOI: https://doi.org/10.33474/j.sa.v3i1.6621

Kurniasih F. Sosialisasi bahaya radikal bebas dan fungsi antioksidan alami bagi kesehatan. [Socialization of the dangers of free radicals and the function of natural antioxidants for health]. J Vokasi. 2019;3(1):1. DOI: https://dx.doi.org/10.30811/vokasi.v3i1.960 DOI: https://doi.org/10.30811/vokasi.v3i1.960

Musyarifah Z, Agus S. Proses fiksasi pada pemeriksaan histopatologik. [Fixation process on histopathological examination]. J Kesehat Andalas. 2018;7(3):443. DOI: https://doi.org/10.25077/jka.v7i3.900 DOI: https://doi.org/10.25077/jka.v7i3.900

Sulistyowati E, Hsu JH, Cheng YB, Chang FR, Chen YF, Yeh JL. Indonesian herbal medicine prevents hypertension-induced left ventricular hypertrophy by diminishing NADPH oxidasedependent oxidative stress. Oncotarget. 2017;8(49):86784–98. DOI: 10.18632/oncotarget.21424 DOI: https://doi.org/10.18632/oncotarget.21424

Masterclass CMEM. Hypertrophic-cardiomyopathy.indd. 2019;19(1):61–3. DOI: https://doi.org/10.7861/clinmedicine.19-1-61 DOI: https://doi.org/10.7861/clinmedicine.19-1-61

Khansa A, Partiningrum DL. Hubungan antara lama hipertensi dan gambaran. Elektrokardiogram hipertrofi ventrikel kiri dan infark miokard lama. [Relationship between duration of hypertension and features. Electrocardiogram of left ventricular hypertrophy and old myocardial infarction]. Jurnal Kedokteran Diponegoro. 2018;7(2):1251–65. DOI: https://doi.org/10.14710/dmj.v7i2.21198

Durrotul Hasanah, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Islam Malang, East Jawa, Indonesia

Nour Athiroh Abdoes Sjakoer, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Islam Malang, East Jawa, Indonesia

Nurul Jadid Mubarakati, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Islam Malang, East Jawa, Indonesia