Effect of Bay Leaf (Syzygium polyanthum) Extract on Antioxidant Activity, MDA Levels, and Liver Histopathology Feature of Ethambutol Induced Wistar Rats


  • Bambang Edi Suwito Department of Anatomy Histology, Universitas Nahdlatul Ulama, Surabaya, Indonesia
  • Lea Maera Shanty Department of Internal Medicine, RSI Jemursari Surabaya, Indonesia
  • Retna Gumilang Department of Parasitology, Universitas Nahdlatul Ulama, Surabaya, Indonesia
  • Handayani Handayani Department of Medical Pharmacology, Universitas Nahdlatul Ulama, Surabaya, Indonesia
  • Renata Alya Ulhaq Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia




Antioxidant, Histopathology, MDA


Bay leaf extract (Syzygiun polianthum) is one herbal elemnt that may be used to lessen liver function issues, lessen symptops of nausea, vomiting, discomfort and improve adherence and the effectiveness of tuberculossi treatment. The objective of this study was to determine the effect of bay leaf (Syzygium polyanthum) extract on antioxidant activity, Malondialdehyde (MDA) levels, and liver histopathology of ethambutol-induced Wistar rats. This research is a laboratory experiment. Antioxidant activity of Bay Leaf Extract was evaluated by comparing vitamin C with spectrophotometry methods. White Wistar rats were separated into 6 groups and uset to test the lebel of MDA and livers histopathology. Group 1 serves as the control group and received DMSO (placebo); Group 2 was received ethambutol 50mg/kg BW; Group 3 was received ethambutol and silymarine with dose 50mh/kg BW. Group 4-6 had been given ethambutol and extra ethanol extract of bay leaves of 75,150, dan 300mg/kg BW, respectively. The results of the spectrophotometry showed that the Bay Leaf Extract had antioxidant activity comparable to that of vitamin C, with an IC50 of 11.4 g ± (4.4%). One-Way ANOVA test results obtained p = 0.002 (p<0.05) while the Kruskal-Wallis test results obtained the Asymp. Sig value 0.105> 0.05. There was a significance difference in each group’s MDA levels (p=0.002). Although there was no significant difference in the liver histopathology of treated rats (p>0.05). While bay leaf extract significantly lowers MDA levels in ethambutol-induced Wistar rats, it has no discernible impact on the liver histopathology of ethambutol-induced Wistar rats. Bay leaf extract possesses antioxidant activity comparable to vitamin C.


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MacNeil A, Glaziou P, Sismanidis C, Date A, Maloney S, Floyd K. Global Epidemiology of Tuberculosis and Progress Toward Meeting Global Targets — Worldwide, 2018. MMWR Morb Mortal Wkly Rep. 2020 Mar;69(11):281–5. https://doi.org/10.15585/mmwr.mm6911a2

Lei S, Gu R, Ma X. Clinical perspectives of isoniazid-induced liver injury. Vol. 5, Liver Research. 2021. p. 45–52. https://doi.org/10.1016/j.livres.2021.02.00

Pennington KM, Kennedy CC, Chandra S, Lauzardo M, Brito MO, Griffith DE, et al. Management and diagnosis of tuberculosis in solid organ transplant candidates and recipients: Expert survey and updated review. J Clin Tuberc Other Mycobact Dis. 2018 May;11(January):37–46. https://doi.org/10.1016/j.jctube.2018.04.001

Cao J, Mi Y, Shi C, Bian Y, Huang C, Ye Z, et al. First-line anti-tuberculosis drugs induce hepatotoxicity: A novel mechanism based on a urinary metabolomics platform. Biochem Biophys Res Commun. 2018 Mar 4;497(2):485–91. https://doi.org/10.1016/j.bbrc.2018.02.030

Dewijanti I, Mangunwardoyo W, Astari D, Hanafi M, Artanti N. Short communication: Effects of the various source areas of Indonesian bay leaves (Syzygium polyanthum) on chemical content and antidiabetic activity. Biodiversitas J Biol Divers. 2020 Feb;21(3):1190–5. https://doi.org/10.13057/biodiv/d210345

Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines. 2018 Aug;5(3):93. https://doi.org/10.3390/medicines5030093

Sahlan M, Rizka Alia Hapsari N, Diah Pratami K, Cahya Khayrani A, Lischer K, Alhazmi A, et al. Potential hepatoprotective effects of flavonoids contained in propolis from South Sulawesi against chemotherapy agents. Saudi J Biol Sci. 2021;28(10):5461–8. https://doi.org/10.1016/j.sjbs.2021.08.022

Hartanti L, Yonas SMK, Mustamu JJ, Wijaya S, Setiawan HK, Soegianto L. Influence of extraction methods of bay leaves (Syzygium polyanthum) on antioxidant and HMG-CoA Reductase inhibitory activity. Heliyon. 2019 Apr 1;5(4):e01485. https://doi.org/10.1016%2Fj.heliyon.2019.e01485

Singletary K. Bay Leaf: Potential Health Benefits. Nutrition Today. 2021;56(4):202-208 https://doi.org/10.1097/NT.0000000000000493

Atmakusuma TD, Nasution IR, Sutandyo N. Oxidative Stress (Malondialdehyde) in Adults Beta-Thalassemia Major and Intermedia: Comparison Between Before and After Blood Transfusion and Its Correlation with Iron Overload. Int J Gen Med. 2021; 14: 6455–6462. https://doi.org/10.2147/ijgm.s336805

Wulandari DD, Mufidah Z, Ersam T, Wulansari DD, Corpuz AV. Alpha mangosteen effect on mda level and the pancreatimorphology rattus norvegicus (Berkenhout, 1769) induced by alloxan. Proc Pakistan Acad Sci Part B. 2020;57(4):15–21. Retrieved from https://ppaspk.org/index.php/PPAS-B/article/view/313

Kristiana R, Pradini A, Indah KM. Efek Ekstrak Etanol Daun Salam (Syzygium polyanthum) Terhadap Hepar Mencit (Mus musculus) Galur DDY pada Uji Toksisitas Akut. [Effects of Bay Leaf (Syzygium polyanthum) Ethanol Extract on the Liver of Mice (Mus musculus) DDY Strain in Acute Toxicity Test]. Med Kartika J Kedokt dan Kesehat. 2021;4(Volume 4 No 5):493–506. https://doi.org/10.35990/mk.v4n5.p493-506

Afifah AN, Dono ND, Zuprizal. Optimizing antioxidant properties of salam (Syzygium polyanthum) leaf extract through nanoencapsulation technology. In: IOP Conference Series: Earth and Environmental Science. 2021. http://dx.doi.org/10.1088/1755-1315/686/1/012040

Brinza I, Boiangiu RS, Hancianu M, Cioanca O, Orhan IE, Hritcu L. Bay Leaf (Laurus Nobilis L.) Incense Improved Scopolamine-Induced Amnesic Rats by Restoring Cholinergic Dysfunction and Brain Antioxidant Status. Antioxidants (Basel) 2021 8;10(2):259. https://doi.org/10.3390/antiox10020259

Widyawaty LE, Nyoman Ehrich Lister I, Lie S. Antioxidant Activity, Total Phenol, and Total Flavonoid of Syzygium polyanthum. In: InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences. 2021. p. 1–5. https://doi.org/10.1109/InHeNce52833.2021.9537187

Morales M, Munné-Bosch S. Malondialdehyde: Facts and artifacts. Vol. 180, Plant Physiology. 2019. p. 1246–50. https://doi.org/10.1104/pp.19.00405

Islam MR, Islam MR, Ahmed I, Moktadir A Al, Nahar Z, Islam MS, et al. Elevated serum levels of malondialdehyde and cortisol are associated with major depressive disorder: A case-control study. SAGE Open Med. 2018;6:205031211877395. https://doi.org/10.1177%2F2050312118773953

Sekiou O, Bouziane I, Bouslama Z, Djemel A. In-Silico Identification of Potent Inhibitors of COVID-19 Main Protease (Mpro) and Angiotensin Converting Enzyme 2 (ACE2) from Natural Products: Quercetin, Hispidulin, and Cirsimaritin Exhibited Better Potential Inhibition than Hydroxy-Chloroquine against. ChemRxiv. 2020;2(April). https://doi.org/10.26434/chemrxiv.12181404.v1

Sulistiyani, Falah S, Wahyuni WT, Sugahara T, Tachibana S, Syaefudin. Cellular mechanism of the cytotoxic effect of extracts from Syzygium polyanthum leaves. Am J Drug Discov Dev. 2014 Mar;4(2):90–101. https://dx.doi.org/10.3923/ajdd.2014.90.101

Hasan R, Lindarto D, Siregar GA, Mukhtar Z. (2020). The effect of bay leaf extract Syzygium polyanthum (Wight) Walp. on C-reactive protein (CRP) and myeloperoxidase (MPO) level in the heart of rat model of myocardial infarction. Medicinski glasnik: official publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina, 17(1), 41–45. https://doi.org/10.17392/1068-20




How to Cite

Suwito, B. E., Shanty, L. M., Gumilang, R., Handayani, H. and Ulhaq, R. A. (2022) “Effect of Bay Leaf (Syzygium polyanthum) Extract on Antioxidant Activity, MDA Levels, and Liver Histopathology Feature of Ethambutol Induced Wistar Rats”, Indonesian Journal of Medical Laboratory Science and Technology, 4(2), pp. 148–156. doi: 10.33086/ijmlst.v4i2.2471.