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Sari Eka Pratiwi Sri Nuryani Wahyuningrum Rachmagreta Perdana Putri Danarto Danarto Didik Setyo Heriyanto Nur Arfian Sofia Mubarika Haryana Indwiani Astuti

Abstract

Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.

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How to Cite
Pratiwi, S. E., Wahyuningrum, S. N. ., Putri, R. P. ., Danarto, D., Heriyanto, D. S. ., Arfian, N., Haryana, S. M. . and Astuti, I. (2022) “MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia”, Indonesian Journal of Medical Laboratory Science and Technology, 4(1), pp. 1–9. doi: 10.33086/ijmlst.v4i1.2355.
Section
Articles
BPH, HGPIN, miR-141, PCA, prostate anomaly markers

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Sari Eka Pratiwi, Department of Biology and Pathobiology, Faculty of Medicine, Universitas Tanjungpura, Indonesia

Sri Nuryani Wahyuningrum, Magelang Unit of Health Research and Development, Ministry of Health, Indonesia

Rachmagreta Perdana Putri, Department of Histology, Faculty of Medicine, Universitas Ahmad Dahlan, Yogyakarta, Indonesia

Danarto Danarto, Department of Urology, Sardjito Hospital, Yogyakarta, Indonesia

Didik Setyo Heriyanto, Department of Anatomy Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Nur Arfian, 6Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Sofia Mubarika Haryana, Department of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Indwiani Astuti, Department of Pharmacology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia