Pigeonpea (Cajanus cajan L.) and Cowpea (Vigna unguiculata L.) Water as Alternative Growth Medium for Escherichia coli and Staphylococcus aureus in Laboratory with Minimum Infrastructure
Article Sidebar
Main Article Content
Abstract
The availability of non-synthetic media from natural ingredients is needed to answer the needs in laboratories where the price of nutrient media is quite expensive and there are limited supplies of material ware houses. Cowpea (Vigna unguiculata L.) and pigeonpea (Cajanus cajan L.) are the local foods of people of NTT (East Nusa Tenggara) which have a high enough nutritional content which has the potential to be developed into cheap, easy and simple non-synthetic media in making. The purpose of this study was to determine whether the agar media contained nutrient from cowpea and pigeonpea water can be used as a alternative for nutrient agar for the growth of Escherchia coli and Staphylococcus aureus bacteria. This research is a true experiment with posttest-only control design. The growth rate of S. aureus bacteria on pigeonpea medium, cowpea medium, nutrient agar medium, were 164 CFU/mL (SD=3,13), 161 CFU/mL (SD=3,02) and 164 CFU/mL (SD=3,21), respectively. The average growth of E. coli on cowpea medium, pigeonpea medium, and nutrient agar control medium were 163 CFU/mL (SD=2,79), 167 CFU/mL (SD-2,63) and 164 CFU/mL (SD=2,75) respectively. Test results ANOVA between pigeonpea medium, cowpea medium and nutrient medium in order to obtain p value = 0.145 (p> 0.05) for the growth of E. coli bacteria and p value = 0.393 (p> 0.05) for growth S. aureus. It was concluded that there was no difference between the number of bacterial colonies of E. coli and S. aureus on three medium. The pigeonpea medium and cowpea can be used to grow and alternative nutrient agar in order to grow bacteria E. coli and S. aureus.
Downloads
Article Details
Copyright (c) 2021 Norma Kambuno, Ninda P. Y. Amtaran, Sherly Dewu, Kuntum E. Nurdin, Ni Made Susilawati, Yoan Novicadlitha
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Fachraniah, Fardiaz D, Idiyanti T. Peptone production from soybean press cake and yeast by papain enzyme for the bacterial growth media. J Teknol dan Ind Pangan. 2002;XIII(3):260–6.
Wulan R, Meryandini A, Sunarti TC. The potential of tapioca industrial liquid waste as a growth media for starter lactic acid bacteria Pediococcus pentosaceus E.1222. J Sumberd Hayati. 2017;3(1):27–33.
Danela S, Gede LS, Ariami P. Soybeans as an alternative media for Pseudomonas aeruginosa bacterial growth bakteri. J Anal Med Biosains. 2019;6(1):73.
Juriah S, Sari WP. Utilization of tofu industrial liquid waste as an alternative media for the growth of Bacillus sp. J Anal Kesehat Klin Sains [Internet]. 2018;6(1):24–9. Available from: http://jurnal.univrab.ac.id/index.php/klinikal/article/view/525/361
Kurniasih N, Rosahdi TD. Comparison of the effectiveness of red bean and green bean extracts as growth media for Lactobacillus acidophilus. Pros Semin Nas Sains dan Teknol Nukl. 2013;212–6.
Tharmila S, Jeyaseelan EC, Thavaranjit AC. Preliminary screening of alternative culture media for the growth of some selected fungi. Arch Appl Sci Res. 2011;3(3):389–93.
Susilawati NM, Kambuno NT, Saputri YI, I Gede Putu Arnawa. Prevalence of negative strain Gram bacteria extended spectrum beta lactamase ( ESBL ) in Rsud Prof . Dr . W . Z . Johannes Kupang. Pakistan J Med Heal Sci. 2020;14(2):1434–6.
Haddish K. Production of single cell protein from fruit of beles (Opuntia ficus-indica L.) Peels Using Saccharomyces cerevisiae. J Microbiol Exp. 2015;2(7).
Nurdin KE, Olla LRY, Feoh SF, Galla ADP, Istnaini KD, Jonison EPF, et al. Effectivity test of 96% from soe (Citrus sinensis L.) sweet orange rind ethanol extract as biolarvaside. J Info Kesehat. 2019;17(2):176–83.
Noriko N. Potency of tea leaf (Camellia sinensis) and Acalypha indica L. earring leaf in inhibiting the growth of Salmonella typhi. J Al-AZHAR Indones SERI SAINS DAN Teknol. 2013;2(2):104.
Dewu S, Bala R, Kambuno NT, Kupang PK. Differences of preparation examination methods for the number of acid-resistant bacteria. 2020;18:59–67.
Anisah A. Alternative Media for bacterial growth using different carbohydrate sources. Vol. 53, Universitas Muhammadiyah Surakarta. 2015.
Safitri N, Sunarti TC, Maeryandini A. Pediococcus pentosaceus lactic acid bacteria growth media formula using tofu whey substrate. J Sumberd Hayati. 2017;2(2):31–8.
Saputra D, Nurhayati T. Production and application of fish peptone for bacterial growth media. J Pengolah Has Perikan Indones. 2014;16(3):215–23.
Indrayana FF. Alternative media for nutrient agar (AN) sweet corn cob waste flour (Zea mays) for the growth of Escherichia coli and Staphylococcus aureus. Poltekkes Kemenkes Bandung. 2020;53(9):1689–99.
Jannah ANAM. Utilization of sweet potato (Ipomoea batatas) Cilembu as a substitute for carbohydrates in potato dextrose agar (PDA) for the growth of Trichophyton rubrum. 2020.
Rosidah U. Tofu dregs flour as a growth medium for Serratia marcescens bacteria. Skripsi Unimus. 2016;1–63.
Al-azzauy AAM, Hassan AM. The beetroot juice as a bacterial growth and maintenance medium for many pathogenic bacteria. Univ Al-Mustansiriyah, Coll Pharm Dept Med Microbiol Biotechnol. 2011;5(3):147–61.
Deivanayaki, M. ; Antony IP. Alternative vegetable nutrient source for microbial growth. Int J Biosci. 2012;2(5):47–51.
Sundari, Wisrakarmila, Marlina D, Faizah. utilization of arum manis (Mangifera indica L.) seeds as alternative growth media for Candida albicans and Aspergillus sp. J Pengelolaan Lab Pendidik. 2021;3(1):14–7.
Purwati S. Utilization of different carbohydrate sources (suweg bulbs, taro bulbs, and kimpul bulbs) as substitution of Na (Nutrient Agar) media for bacterial growth. J Chem Inf Model. 2013;53(9):1689–99.
Khaerunnisa, Rismaya, Iis K, Dewi N, Asep D. Utilization of boiled water of yellow and purple bulbs as alternative media for the growth of Escherichia coli and Staphylococcus aureus. J Ris Kesehat Poltekkes Depkes Bandung. 2019;11(1):269.
Widya A. Growth of Escherichia coli and Bacillus subtilis bacteria on cassava, white sweet potato and yellow sweet potato media as substitute for NA media. Skripsi. 2016;1–13.
Rizki Z, Syahnitya H. Utilization media bengkuang (Pachyrrhizus erosus) and bean sprouts (Vigna radiate) as alternative media for Escherichia coli and Staphylococcus aureus bacterial growth. Sel J Penelit Kesehat. 2019;6(1):1–9.
Rahmawati R. Fungal growth in kluwih seed media and jackfruit seeds as substitute for PDA media. 2016;2016.
Rahma Faradiana. Utilization of Different carbohydrate sources (Suweg Bulbs and Kimpul Bulbs) as substitutes for PDA media for fungal growth. Skripsi [Internet]. 2019;53(9):1689–99. Available from: www.journal.uta45jakarta.ac.id
Puspita D, Palimbong S, Pratamaningtyas NL, Nugroho KPA. Proximate Analysis of different types of legumes growing on the island of Timor-NTT. Semin Nas Tek Kim Kejuangan. 2017;0(0):05.
American Public Health Association, American Chemical Society A of OAC (U. S. Standard methods for the examination of water and sewage. In: Association of Official Agricultural Chemists (US). 1920.
Martyniuk S, Oroń J. Use of potato extract broth for culturing root-nodule bacteria. Polish J Microbiol. 2011;60(4):323–7.
