Liver Histopathological Change and Malondialdehyde Level of Rattus Norvegicus on Administration of Curcuma Zedoaria and Paracetamol Toxic Dose
Article Sidebar
Main Article Content
Abstract
High doses of paracetamol create necrosis in the liver and produce free radicals. When liver function decreased in a long time, it will lead to severe liver damage and it will be irreversible. Rhizome of Curcuma zedoaria has the potential effect as an antioxidant, it is assumed that its properties inhibit the formation of free radicals which formed from toxic doses of paracetamol. The aim of this study was to examine the histological structure of the liver and to determine MDA levels in the administration of C. zedoaria toxic dose and paracetamol on the Rattus norvegicus. The study was used twenty-four rats divided into four groups (positive control: CMC 0.5%; negative control: paracetamol 1.35g/kg body weight; treatment group 1 (T1): C. zedoaria 105 mg/200g and paracetamol 1.35g/kg Body weight 2 hours later, and treatment group 2 (T2): paracetamol 1.35g/ kg Body weight and C. zedoaria 105 mg/200g 2 hours later). The Kruskall-Wallis test results showed MDA level did not significantly different between groups (p = 0.087). Hepatocellular changes were observed descriptively with Hematoxylin-eosin staining. Positive control showed greater hepatocellular changes rather than other groups, hepatocyte cells were enlarged with cytoplasm showing eosinophilic granules infiltrates, enlarged irregular nuclei, nucleolus prominent, there are many necrosis cells.
Downloads
Article Details
Copyright (c) 2021 Putu Oky Ari Tania, Puja Ayu Misuari, Satya Yudhayana, Ketut Ayesha Edelwise Prayoga
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Rahman A, Afroz M, Islam R, Islam KD, Amzad Hossain M, Na M. In vitro antioxidant potential of the essential oil and leaf extracts of Curcuma zedoaria Rosc. J Appl Pharm Sci. 2014;4(2):107–11.
Ji S, Fattahi A, Raffel N, Hoffmann I, Beckmann MW, Dittrich R, et al. Antioxidant effect of aqueous extract of four plants with therapeutic potential on gynecological diseases; Semen persicae, Leonurus cardiaca, Hedyotis diffusa, and Curcuma zedoaria. Eur J Med Res [Internet]. 2017;22(1):1–8. Available from: https://doi.org/10.1186/s40001-017-0293
Marliani L, Budiana W, Anandari Y. The Effect of Extraction Condition on The Polyphenol Content and Antioxidant Activity of Curcuma zedoaria (Christm.) Roscoe Rhizome. Indones J Pharm Sci Technol. 2017;4(2):57.
Azahar NF, Gani SSA, Mohd Mokhtar NF. Optimization of phenolics and flavonoids extraction conditions of Curcuma Zedoaria leaves using response surface methodology. Chem Cent J. 2017;11(1):1–10.
Zahra U. Short Communication: Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent. Nusant Biosci. 2016;8(2):221–5.
Phaniendra A, Jestadi DB, Periyasamy L. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian J Clin Biochem. 2015;30(1):11–26.
Aycan IÖ, Tüfek A, Tokgöz O, Evliyaoǧlu O, Firat U, Kavak GÖ, et al. Thymoquinone treatment against acetaminophen-induced hepatotoxicity in rats. Int J Surg. 2014;12(3):213–8.
Tittarelli R, Pellegrini M, Scarpellini MG, Marinelli E, Bruti V, Di Luca NM, et al. Hepatotoxicity of paracetamol and related fatalities. Eur Rev Med Pharmacol Sci. 2017;21(1):95–101.
Kaushik M, Jalalpure S. Effect of Curcuma zedoaria Rosc root extracts on behavioral and radiology changes in arthritic rats. J Adv Pharm Technol Res. 2011;2(3):170.
Amin F, Yanti NI, Sartini S, Sumarheni S. Efek Pemberian Ekstrak Etanol Rimpang Temu Putih (Curcuma Zedoaria (Berg.) Roscoe) Terhadap Perubahan Kadar Protein Total Dan Alkali Fosfatase Pada Tikus (Rattus Norvegicus) Yang Dipaparkan Asap Rokok. Maj Farm Dan Farmakol. 2019;22(3):99–103.
Hadinata, Teguh M, Nurhayati. Uji Efek Hepatorepair Ekstrak Temulawak (Curcuma xanthorrhiza Roxb) pada Tikus Putih Jantan Galur Wistar yang Diinduksi Paracetamol (Skripsi) [Test of the Hepatorepair Effect of Temulawak Extract (Curcuma xanthorrhiza Roxb) on Wistar Male White Rats Induced by Paracetamol]. Surakarta: Fakultas Kedokteran Universitas Muhammadiyah Surakarta; 2016.
Rivera P, Pastor A, Arrabal S, Decara J, Vargas A, Sánchez-Marín L, et al. Acetaminophen-induced liver injury alters the Acyl ethanolamine-based anti-inflammatory signaling system in liver. Front Pharmacol. 2017;8(OCT):1–16.
Ratziu V, Harrison SA, Francque S, Bedossa P, Lehert P, Serfaty L, et al. Elafibranor, an Agonist of the Peroxisome Proliferator-Activated Receptor-α and -δ, Induces Resolution of Nonalcoholic Steatohepatitis Without Fibrosis Worsening. Gastroenterology. 2016;150(5):1147-1159.e5.
Hamza RZ, Al-Harbi MS. Amelioration of paracetamol hepatotoxicity and oxidative stress on mice liver with silymarin and Nigella sativa extract supplements. Asian Pac J Trop Biomed [Internet]. 2015;5(7):521–31. Available from: http://dx.doi.org/10.1016/j.apjtb.2015.03.011
Hemabarathy B, Budin SB, Feizal V. Paracetamol hepatotoxicity in rats treated with crude extract of Alpinia galanga. J Biol Sci. 2009;9(1):57–62.
Merdana IM, Watiniasih NL, Sudira IW, Samsuri. The Effect of Ethanol Extract Mymercodia pendans on Paracetamol-Induced Hepatotoxicity in White Rats. IOP Conf Ser Earth Environ Sci. 2019;248(1).
Dogaru G, Bulboaca AE, Gheban D, Boarescu PM, Rus V, Festila D, et al. Effect of liposomal curcumin on acetaminophen hepatotoxicity by down-regulation of oxidative stress and matrix metalloproteinases. In Vivo (Brooklyn). 2020;34(2):569–82.
O’Brien PJ, Slaughter MR, Swain A, Birmingham JM, Greenhill RW, Elcock F, et al. Repeated acetaminophen dosing in rats: Adaptation of hepatic antioxidant system. Hum Exp Toxicol. 2000;19(5):277–83.
Sayed MM, El-Kordy EA. The protective effect of curcumin on paracetamol-induced liver damage in adult male rabbits: Biochemical and histological studies. Egypt J Histol. 2014;37(4):629–39.
Swaroop TVSS, Gowda S. Hepatotoxicity Mechanisms and its Biomarkers. Int J Pharm Chem Sci. 2012;1(2):675–82.
Hamid A, Lee LS, Karim SR, Jufri NF. Hepatoprotective effects of zerumbone against paracetamol-induced acute hepatotoxicity in rats. Malaysian J Med Sci. 2018;25(2):64–71.
Eugenio-Pérez D, Montes de Oca-Solano HA, Pedraza-Chaverri J. Role of food-derived antioxidant agents against acetaminophen-induced hepatotoxicity. Pharm Biol. 2016;54(10):2340–52.
