Electrolytes Levels (Na, K, Cl) in Serum Stored at 4°C Temperature
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Abstract
The samples used for serum electrolyte measurement should be analyzed immediately after being received in the laboratory within 1-2 hours to avoid an increase in the error of the results. Serum should be stored at 4°C for a period to prevent damage. The analyst should consider maximum delay time in the examination to maintain the serum's quality. This study compared the 2-hour and 3-hour delays in sodium (Na), potassium (K), and chlorine (Cl) tests. The method used in this study is an observational analysis with a cross-sectional study design. The samples in this study used 35 patient serum residues. The study was conducted in November 2020 with a continuous sampling technique. Electrolyte levels in the sample were measured by AVL 9180 Electrolyte Analyzer using Ion-Selective Electrode (ISE) method. The differences in electrolyte (Na, K, Cl) levels were analyzed by the Kruskal-Wallis Statistical test at a 95% confidence level. The results showed that the content of sodium, potassium, and chlorine were 0.719; 0.976; and 0.772. This study showed that there was no significant difference in the electrolyte content of sodium (Na), potassium (K), and chlorine (Cl) in the serum directly detected from the serum stored at 4°C for 2 hours and 3 hours. In conclusion, it is acceptable to postpone the serum test for 3 hours with various considerations.
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Copyright (c) 2021 Amalia Nurul Fauziah, M. Atik Martsiningsih, Budi Setiawan
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References
Capasso G, Unwin R. Electrolytes and acid–base: common fluid and electrolyte disorders. Medicine (Baltimore) [Internet]. 2011 Jun;39(6):317–24. Available from: https://linkinghub.elsevier.com/retrieve/pii/S1357303911000636
Rahmawati F. Aspect of chronic kidney disease. J Ilm Kedokt Wijaya Kusuma. 2018;
Makani M, Setyaningrum N. Patterns of furosemide use and electrolyte imbalance in heart failure patients at Hospital X Yogyakarta. J Ilm Farm. 2017;
Tyas RA, Damayanti W, Arguni E. Prevalence of serum electrolyte disorder in children under five with diarrhea and dehydration in Dr Sardjito Hospital on 2013-2016. Sari Pediatr. 2018;
Kusnanto. Modul learning modules meeting fluid and electrolyte needs. Fakultas Keperawatan Universitas Airlangga. 2016.
Yaswir R, Ferawati I. Physiology and balance disorders of sodium, potassium and chloride and laboratory examination. J Kesehat Andalas. 2012;
Horne M dan PS. Pocket guide to fluids, electrolytes, and acid-base balance. 2nd ed. Asih Y, editor. Jakarta: EGC; 2012.
Pfortmueller CA, Uehlinger D, von Haehling S, Schefold JC. Serum chloride levels in critical illness—the hidden story. Intensive Care Med Exp. 2018;
Salam SH. The Basics of fluid and electrolyte therapy. Bahan Kuliah FK Unhas. 2016;
Plebani M. Quality indicators to detect pre-analytical errors in laboratory testing. Clinical Biochemist Reviews. 2012.
Lippi G, Chance JJ, Church S, Dazzi P, Fontana R, Giavarina D, Grankvist K, Huisman W, Kouri T, Palicka V, Plebani M, Poro V, Salvagno GL, Sndberg S, Sikaris K, Watson I, Stankovic AK, Simundic AM. Preanalytical quality improvement: From dream to reality. Clin Chem Lab Med. 2011 Jul;49(7):1113-26.
Baruah A, Goyal P, Sinha A, Ramesh KL, Datta R. Delay in specimen processing-major source of preanalytical variation in serum electrolytes. J Clin Diagnostic Res. 2014;
Trisna YN, Mutmainnah M, DN Pakasi R, Hardjoeno H. Na, K, Cl concentration in time interval examination variations of serum. Indones J Clin Pathol Med Lab. 2018;
Balci AK, Koksal O, Kose A, Armagan E, Ozdemir F, Inal T, Oner N. General characteristics of patients with electrolyte imbalance admitted to emergency department. World Journal of Emergency Medicine. 2013;4(2):113-6
Arneson W. Electrolytes: The salts of the earth. Laboratory Medicine. 2014.
Barth JH. Clinical quality indicators in laboratory medicine. Ann Clin Biochem. 2012;
Roumelioti M-E, Glew RH, Khitan ZJ, Rondon-Berrios H, Argyropoulos CP, Malhotra D, et al. Fluid balance concepts in medicine: Principles and practice. World J Nephrol. 2018;
Hedayati M, Razavi SA, Boroomand S, Kheradmand Kia S. The impact of pre-analytical variations on biochemical analytes stability: A systematic review. Journal of Clinical Laboratory Analysis. 2020.
Donnelly JG, Soldin SJ, Nealon DA, Hicks JM. Stability of twenty-five analytes in human serum at 22 degrees C, 4 degrees C, and -20 degrees C. Pediatr Pathol Lab Med. 1995;
O’Keane MP, Cunningham SK. Evaluation of three different specimen types (serum, plasma lithium heparin and serum gel separator) for analysis of certain analytes: clinical significance of differences in results and efficiency in use. Clin Chem Lab Med [Internet]. 2006 Jan 1;44(5). Available from: https://www.degruyter.com/view/j/cclm.2006.44.issue-5/cclm.2006.099/cclm.2006.099.xml
Dupuy AM, Cristol JP, Vincent B, Bargnoux AS, Mendes M, Philibert P, et al. Stability of routine biochemical analytes in whole blood and plasma/serum: focus on potassium stability from lithium heparin. Clin Chem Lab Med [Internet]. 2018 Feb 23;56(3):413–21. Available from: https://www.degruyter.com/doi/10.1515/cclm-2017-0292
Scott MG, LeGrys VA, Hood JL. Electrolytes and blood gases. in: tietz textbook of clinical chemistry and molecular diagnostics. 2012.
Burger P, Kostova E, Bloem E, Hilarius-Stokman P, Meijer AB, van den Berg TK, et al. Potassium leakage primes stored erythrocytes for phosphatidylserine exposure and shedding of pro-coagulant vesicles. Br J Haematol. 2013;
Apriliani I, Santosa B, Sukeksi A. Difference in electrolyte levels (Na, K, Cl) in samples immediately and delayed by 150 minutes. repository.unimus.ac.id. 2018.
Azizah N, Aliviameita A. Effect of delay of serum examination on electrolyte levels of sodium and chloride. J Med Lab Sci Technol. 2019;
An B, Park C-E. Evaluation of stability of serum on different storage temperatures for routine chemistry analytes. Korean J Clin Lab Sci. 2014;
Departemen Kesehatan RI. Pedoman praktek laboratorium yang benar (Good laboratory practice). Jakarta; 2008.
