The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus
Article Sidebar
Main Article Content
Abstract
Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).
Downloads
Article Details
Copyright (c) 2021 Julia Nanda Puspita, Rikhsan Kurniatuhadi, Rahmawati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Arenz S, Wilson DN. Blast from the past: reassessing forgotten translation inhibitors, antibiotic selectivity, and resistance mechanisms to aid drug development. Mol Cell. 2016;61(1):1-12.
Amalia S, Wahdaningsih S, Untari EK. Activity testdragon fruit skin n-hexane fraction antibacterial red (Hylocereus polyrhizus Britton & Rose) against Staphylococcusaureus ATCC 25923. Pontianak: Faculty Tanjungpura University Medicine. J Phy Ina. 2016; 61-64.
Entjang I. Microbiology and parasitology for the nursing academy and health power school of the equals. PT. Citra Aditya Bakti; 2018: 56-58
Brooks GF, Karen CC, Butel JS, Stephen AM. Jawetz, Melnick & Adelberg’s, Book 1 Medical Microbiology. Jakarta: EGC; 2015.
Plata FX, Ebergeny X, Resendiz JL, Villarreal O, Barcena R, Viccon JA, Mendoza GD. Palatability and chemical composition of feeds ingested in captivity by Yucatan white-tailed deer (Odocoileus virginianus yucatanensis). Arc Med Vet. 2019; 123-129.
Manalu J, Rahmawati, Hidayat N. Antifungal activity of Actinomycetes isolates from hot springs Ai Sipatn Lotup Sanggau against isolate Hortaea werneckii (T1). Protobiont. 2019; 69-77.
Jawetz E, Melnick JL, Adelberg EA. Medical microbiology. Translated by Mudihardi E, Kuntaman, Wasito EB, Mertaniasih NM, Harsono S, Alimsardjono L, Edition XXII, 327-335, 362-363, Publisher Salemba Medika, Jakarta; 2015.
Teta R, Marteinsson VT, Longeon A, Klonowski AM, Groben R, Bourguet KM, CostantinoV, MangoniA. Thermoactinoamide A, an antibiotic lipophilic cyclopeptide from the icelandic thermophilic bacterium Thermoactinomyces vulgaris. J Nat Prod. 2017; 2530-2535.
Mulyadi, Sulistyani N. Activity of 12 Actinomycetes isolates against bacteria resistance: Public Health. 2013;7(2): 55-112.
Zheng L, Chen H, Han X, Yan X. Antimicrobial screening and active compound isolation from marine bacterium NJ6-3-1 associated with the sponge Hymeniacidon parleve. World J Microb Biotech. 2015; 201-206.
Moningka KS, Kojong NS, Sudewi S. Antibacterial activity test of leaf extract of cats (Acalypha hispida Burm. F.) against Staphylococcus aureus and Escherichia coli in vitro. J Pharm Sci. 2015; 2302-2493.
Warbung YY, Wowor VNS, Posangi J. Inhibition of sea sponge extract Callyspongia sp. against the growth of Staphylococcus aureus bacteria. EGiGi Journal (eG). 2013; 1 (2): 2.
Bratchkova A, Ivanova V, Gousterova A, Laatsch L. β-Carboline alkaloid constituents from a Thermoactinomyces sp. strain isolated from Livingston Island. Biotechnology & Biotechnological Equipment: Antarctica. 2012: 3005-3009.
Todar, K. Pathogenic E. coli, Todar’s Online Textbook and Bacteriology, http://textbookofbacteriology.net/e.coli.html, 2018.
Liu J. Minimum Effective Dose. Encyclopedia of biopharmaceutical statistics. Taylor & Francis. p. 1493. doi:10.1081/E-EBS3-130001128. 2019. ISBN 978-1-4398-2246-3.
Limaye L, Patil R, Ranadive P, Kamath G. Application of potent actinomycete strains for bio-degradation of domestic agro-waste by composting and treatment of pulp-paper mill effluent. Adv Micro. 2017; 94-108.
Gunawan I. Preliminary extraction of bioactive compounds as antibacterials as well as toxicity test and Minimum Inhibitor Concentration (MIC) test of soft rocks of origin perairan pulau pulau, kepulauan seribu. Description: Faculty of Fisheries and Marine Science, Bogor. 2019.
Bagg J, Mcfarlane, Wallace T, Poxton, Ian R, Smith, Andrew J. Essentials of microbiology for dental students, 2nd Edition. Oxford University, 2017.
Chaudhary HS, Yadav J, Shrivastava AR, Singh S, Singh KA, Gopalan N. Antibacterial activity of Actinomycetes isolated from different soil samples of Sheopur. J Adv Pharm Technol. 2017; 118–123.
Levinson W. Review of medical microbiology, The McGraw-Hill Companies, 25-26, 78-79, 2018.
Sharma SK, Gupta VK. In vitro antioxidant studies of Ficus racemosa linn root, Pharmacognosy Magazine, India. 2008.
