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Putra Rahmadea Utami Chairani Hendra Yudha


Chinese petai (Leucaena leucocephala folium) and aloe vera (Aloe vera L.) have medicinal properties among the plants.  The objective of this study was to determine the number of inhibitory zones produced by the ethanol extract of Chinese petai and aloe vera on the growth of Escherichia coli. The research method was In-vitro Experimental Laboratory research design with the Kirby Bauer method. The samples used was Chinese petai and aloe vera with pure strains of E. coli. One Way ANOVA was used to compare the differences in inhibition of Chinese petai and aloe vera on the growth of E. coli. The results of a combination of Chinese petai and aloe vera extract test showed that there were significant differences in the concentration of 25 g, 50 g, 75 g, and 100 g (p <0.05). The ethanol extract of Chinese petai and aloe vera can inhibit the growth of E. coli. From the results of this study found that there was an interaction on the combination of ethanol extract of Chinese petai and aloe vera inhibiting the growth of E. coli with the most effective concentration being 100 g/mL. This study can find out the benefits of petai cina and aloe vera also the public will know the benefits and efficacy of Chinese petai and aloe vera leaves in medicine.


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Rahmadea Utami, P., Chairani and Yudha, H. (2020) “COMBINATION TEST OF CHINESE LEAF EXTRACT (Leucaena leucocephala folium) AND ALOE VERA INHIBITING GROWTH Escherichia coli”, Indonesian Journal of Medical Laboratory Science and Technology, 2(2), pp. 60–67. doi: 10.33086/ijmlst.v2i2.1577.
Leucaena leucocephala folium, Aloe vera L., Escherichia coli, inhibition, combination


Utami PR, Chairani C, Ilhamdi I. Interaction of Ethanol Extract of Chinese Petai Leaves (Leucaena leucocephala folium) and Aloe Vera (Aloe vera L.) Inhibits the Growth of Staphylococus aureus by Invitro. J Kesehat PERINTIS (Heal Journal). 2019;6(2):186–92.

Rahardjo M, Koendhori EB, Setiawati Y. Antibacterial Activity Test of Aloe Vera (Aloe vera) Ethanol Extract Against Staphylococcus aureus Bacteria. J Kedokt syiah Kuala. 2017;17(2):65–70.

Ramasubramaniam TS, Sivakumar V, Arasu T. Antimicrobial activity of Aloe vera (L.) Burm. F. against pathogenic microorganisms. J Biosci Res. 2010;1(4):251–8.

Suryati N, Bahar E, Ilmiawati I. Antibacterial Effectiveness Test of Aloe vera Extract Against Escherichia coli Growth In Vitro. J Kesehat Andalas. 2018;6(3):518.

Suhaimi S, Indrawati T, Kumala S. Combination Test of Crude Fruit Extracts (Aloe vera. (L) brum. F.) and Blue Leaves (Piper crocatum ruiz & pav) Extracts for Purple Leaf (Piper crocatum ruiz & pav) Antibacteries for Acne. JIFFK J Ilmu Farm dan Farm Klin. 2018;15(01):12.

Leaves DW, Sartinah A. Identification of Antibacterial Compounds Isolated from Leucaena leucocephala (Lam.) De Wit. Leaves. Tradit Med Pap. 2010;15(3):22–8.

Abo-Youssef AMH, Messiha BAS. Beneficial effects of Aloe vera in treatment of diabetes: Comparative in vivo and in vitro studies. Bull Fac Pharmacy, Cairo Univ [Internet]. 2013;51(1):7–11. Available from:

Sewta CA, Mambo C, Wuisan J. Effect Of Extract Effect of Vocational Leaf Extract (Aloe vera L.) on Healing of Rabbit Skin (Oryctolagus cuniculus) Healing. J e-Biomedik. 2015;3(1):1–7.

Kolenda R, Burdukiewicz M, Schierack P. A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front Cell Infect Microbiol. 2015;5:23.

De Biase D, Lund PA. The Escherichia coli Acid Stress Response and Its Significance for Pathogenesis. Adv Appl Microbiol. 2015;92:49–88.

Yunes Panahi, Hossein Khedmat, Ghasem Valizadegan, Reza Mohtashami AS. Efficacy and safety of Aloe vera syrup for the treatment of gastroesophageal reflux disease: a pilot randomized positive-controlled trial. J Tradit Chin Med [Internet]. 2015;35(6):632–6. Available from:

Haritha K, Ramesh B, Saralakumari D. Effect of Aloe vera gel on antioxidant enzymes in streptozotocin-induced cataractogenesis in male and female Wistar rats. J Acute Med [Internet]. 2014;4(1):38–44. Available from:

Rieuwpassa IE, Rahmat, Karlina. Inhibition of Aloe vera extract on the growth of Staphylococcus aureus (in vitro study) Inhibition of Aloe vera extract on the growing of Staphylococcus aureus (An in vitro study). Dentofasial. 2011;10(2):65–70.

A’lana L, Sari R, Apridamayanti P. Determination of the FICI Value Combination of Aloe vera (Aloe vera) Bark and Aloe vera Leaf Skin. Pharm Sci Res. 2017;4(3):132-142

Indriaty S, Indrawati T, Taurhesia S. Test Activities Of Combination Of Aloe Vera (Aloe vera L.) And Sweet Roots (Glycyrrhiza glabra L.) As Hair. Pharmaciana. 2016;3:55–62.

Asyraf MN, Noviyandri PR, Andayani R, Studi P, Dokter P, Fakultas G, et al. Effect of Aloe Vera Extract on the Growth of Enterococcus faecalis at Various Concentrations. J Caninus Denstistry. 2017;2(November):157–61.

Fani M, Kohanteb J. Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria. J Oral Sci. 2012;54(1):15–21.

Pandey R, Mishra A. Antibacterial activities of crude extract of aloe barbadensis to clinically isolated bacterial pathogens. Appl Biochem Biotechnol. 2010;160(5):1356–61.

Rahmawati. Interaction of Extracts of Aloe Vera (Aloe vera L.) and Sirih Leaves (Piper betle L.) on Invitro Obstacles. J Edubio Trop. 2015;2(1):121–7.

Fiţ NI, Chirilă F, Nadăş G, Pall E, Mureşan R. Comparative testing of antimicrobial activity of aqueous extracts of Aloe vera and Lycium barbarium. Bull Univ Agric Sci Vet Med Cluj-Napoca - Vet Med. 2013;70(1):72–6.

Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. J Tradit Complement Med. 2015;5(1):21–6.

Sheikh M, Malik AR, Meghavanshi MK, Mahmood I. Studies on Some Plant Extracts for Their Antimicrobial Potential against Certain Pathogenic Microorganisms. Am J Plant Sci. 2012;03(02):209–13.

Prasaja D, Darwis W, Astuti S. The Effectiveness Test of Combination of Extracts of Raw Leather and Mangosteen Fruit Skin (Garcinia mangostana L.) as Antibacterial of Shigella dysentriae. J Ilmu Lingkung. 2016;12(2):83.