• Theosobia Grace Orno Department of Health Analyst, Poltekkes Kemenkes Kendari, Kendari, Indonesia
  • Agnes Rantesalu Department of Health Analyst, Poltekkes Kemenkes Kupang, Kupang, Indonesia



Cytotoxicity, Enhalus acoroides, soropia coastal waters, southeast regency


The studies analysing the use of natural ingredients as an alternative treatment in the field of pharmacology are developing very rapidly. One of researches that is quite promising in the pharmaceutical industry is the application of marine materials. Marine materials that are frequently used consist of shellfish, algae, sponges and seagrass. Several studies on toxicity tests have shown that the methanol extract of seagrass (from species Enhalus acoroides) is more toxic than the other seagrass family. This study aims to test the toxicity level of Seagrass (E. acoroides) extract from Soropia coastal waters. The research method in this study was an experimental laboratory using E. acoroides Seagrass as a sample that was obtained from Soropia Coast, Konawe Regency, Southeast Sulawesi Province. The sample was extracted using methanol as a solvent by macerating it and was tested for its toxicity using the Brine Shrimp Lethality Test (BSLT) method. Toxicity test results showed that the samples with a concentration of 10 ppm, 100 ppm and 1000 ppm in leaves extracts produced an LC50 value of 404.88 ppm, while the stem and root extracts has a value of LC50 >1000 ppm. The test was continued with higher concentration of leaves extracts consisted of 250 ppm, 500 ppm, and 1000 ppm. The toxicity test showed an LC50 value of 0.7309; which means that it was very toxic. The methanol extract of Seagrass (E. Acoroides) is potential to be used for further analysis and anticancer formulations.


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Permana AH, Husni A, Budhiyanti SA. Antioxidant Activity and Toxicity of Seagrass Cymodocea Sp. Journal of Agricultural Technology. 2016 Apr;17(1):37-46.

Jaafarzadeh N, Hashempour Y, Angali KA. Acte Toxicity Test Using Cyanide on Daphina magna by Flow-Through System. Journal of Water Chemistry and Technology. 2013;35(6):281-286.

Satria D, Silalahi J, Haro G, Ilyas G, Hsb PAZ. Antioxidant and Antiproliferative Activities of an Ethylacetate Fraction of Picria Fel-Terrae Lour. Herbs. Asian Pacific Journal of Cancer Prevention. 2017;18(2):399-403. Available from: articles/ PMC5454734/

Ahmad A, Usman H, Natsir H, Karim A. Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. 2014;2(5):134-140.

Fajarullah A, Irawan H, Pratomo A. Ekstraksi Senyawa Metabolit Sekunder Lamun 4. Thalassodendron Ciliatum Pada Pelarut Berbeda. Repository UMRAH. 2014.

Puspitasari R. Evaluation of the Use of Seagrass Extract as Active Antifouling Material for Aquatic Producers. Jurnal Segara. 2016 Mar 5;12(1).

Amudha P, Jayalaksmi M, Pushpabharathi N, Vanitha V. Identification of Bioactive Components in Enhalus acoroides Seagrass Extract by Gas Chromatography–Mass Spectrometry. Asian Journal of Pharmaceutical and Clinical Research. 2018;11(10):131-137.

Widiastuti E, Arfiani R, Kairani IA, Christyanto Y, Ara NF, Maharani HW. Antioxidant Effect of Clerodendrum sp and Acanthus illicifolius Methanol Extraction on Blood Profile of Male Mice Induced by Benzo(α)pyrene. The 4th International Conference on Biological Science and Biotechnology. 2019 July:1-9

Permana AH, Husni A, Budhiyanti SA. Antioxidant Activity and Toxicity of Seagrass Cymodocea sp. Extracts. Journal of Agricultural Technology. 2016 Apr 23;17(1):37-46.

Kurnia Y, Widiastuti EL, Nurcahyani E. Effect of Antioxidant Taurine, Seagrass (Enhalus acoroides L.), and Red Algae (Eucheuma cottonii L.) on Oxidative Stress in Some Organ Homeostasis in Which Infected by Glifosat. Proceedings of the National Seminar on Indonesian Medicinal Plants 55th. 2019;1(1):296-303.

Baehaki A, Widiastuti I, Herpandi H. Antioxidant Activity of Extracts of HalodulepinifoliaSeagrass from Solvents with Different Polarities. Oriental Journal of Chemistry. 2017;33(1):181-185.

Wardani G, Farida N, Andayani R, Kuntoro M, Sudjarwo SA. The Potency of Red Seaweed (Eucheuma cottonii) Extracts as Hepatoprotector on Lead Acetate-induced Hepatotoxicity in Mice. Pharmacognosy Research .2017;9(3):282-286.

Rengasamy KRR, Arumugam R, Thangaradjou T, Anantharaan P. Phytochemical Constituents, Antioxidant Properties and P-Coumaric Acid Analysis in Some Seagrasses. Food Research International. 2013;54(1):1229-1236.

Wang XB, Sun ZH, Fan LX, Liu YY, Feng J, Ma GX, Chen DL. Two novel diterpenes from the stems and leaves of tropical seagrass Enhalus acoroides in the South China sea. Natural product research. 2019 Aug 19:1-9.

Aprilyani V, Hamdi A, Arami H. Types of Diversity and Seagrass Distribution Patterns in the Waters of Holimombo Village, Buton Regency. Journal of Water Resource Management. 2018;3(4):309-317.

Wagner JG. Pharmacokinetics for The Pharmaceutical Scientist. Technomic Pub., Lancarter-Basel. 1993.



How to Cite

Orno, T. G. and Rantesalu, A. (2020) “INVITRO CITOTOXICITY ASSAYS OF SEAGRASS (Enhalus acoroides) METHANOL EXTRACT FROM SOROPIA COASTAL WATERS SOUTHEAST SULAWESI REGENCY”, Indonesian Journal of Medical Laboratory Science and Technology, 2(1), pp. 27-33. doi: 10.33086/ijmlst.v2i1.1463.