Main Article Content

Noor Febryani Ikke Nanda Amalia Intan Dwi Anggraeni Gilang Nugraha

Abstract

Hemolysis can significantly affect the reliability of test results and occur in the pre-analytical phase. The aim of this study is to reveals the correlation of hemoglobin levels on hemolysis sample. This experimental study was carried out using samples from thirty students of Medical Laboratory Technology study program of the Nahdlatul Ulama University of Surabaya. Blood samples were treated by hard shaken in 30 times in order to damage the middle part of the blood sample. Data on hemoglobin levels were collected and analyzed using the Pearson Correlation Test before and after treatment with significant value p < 0.05 indicating that there was a significant correlation. Hemoglobin concentrations were strongly positive for the hemolysis of the sample (p = 0.000). The conclusion is that sample hemolysis has a potency to be the confounding factor on the hemoglobin test.

Downloads

Download data is not yet available.

Article Details

How to Cite
Febryani, N. ., Amalia, I. N. ., Anggraeni, I. D. . and Nugraha, G. (2019) “STUDY OF HEMOGLOBIN LEVELS ON HEMOLYSIS SAMPLE”, Indonesian Journal of Medical Laboratory Science and Technology, 1(2), pp. 74–79. doi: 10.33086/ijmlst.v1i2.1311.
Section
Articles
Hemoglobin, sample hemolysis, pre-analytical phase

References

Nugraha G. Laboratory practice guide on basic hematology. Trans Info Media; 2017.

Hernaningsih Y, Akualing JS. The effect of hemolysis on plasma prothrombin time and activated partial thromboplastin time test using photo-optical method. Medicine. 2017; 96 (38): 1-5.

Michael P, Phelan, Edmund Z, Reineks, Jesse, Schold, Frederic, Chamberlin J, Gary, Procop. Pre analytic factors associated with hemolysis in emergency department blood samples. Arch Pathol Lab Med. 2018; 142: 229-235.

Adiga US. Hemolytic index-a tool to measure hemolysis in vitro. IOSR Journal of Biotechnology and Biochemistry. 2016;2(2): 49-52.

Multinovic D, Andrijevic I, Licina M. & Andrijevic L. Confidence Level in Venipuncture and Knowledge on Causes of In Vitro Hemolysis Among Healthcare Professionals. Biochemia Medica. 2015: 25(3): 401-409.

Kazmierczak SC. Accurate results in the clinical laboratory:a guide to error detection and correction. Amsterdam (Netherlands): Elsevier Academic Press; 2019. Chapter 5, Interferences of hemolysis, lipemia and high bilirubin on laboratory tests; 57-65.

Saputro DA, Junaidi S. Giving the vitamin C on physical practice maximum and change the hemoglobin levels and erythrocyte count. 2015; 4(3): 32-40.

Norsiah W. The Difference of Hemoglobin Levels Use Sianmethemoglobin Method With and Without Centrifugation on Leucositosis. 2015; 1(2): 72-83.

Praptomo, AJ. Quality Control Of Medical Laboratory. Deepublish; 2018.

Colvin, H. The Breakdown on Hemolyzed Specimens. 2018; 14(1): 1-2.

Noor Febryani, Science of Biological Resources, The United Graduate School of Agriculture Science, Gifu University, Gifu, Japan

Ikke Nanda Amalia, Department of Basic Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya Indonesia

Intan Dwi Anggraeni, Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia

Gilang Nugraha, Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia