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Nosy Maria Riscahyani Evy Ratnasari Ekawati Khoirul Ngibad

Abstract

Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.

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How to Cite
Riscahyani, N. M. ., Ekawati, E. R. and Ngibad, K. (2019) “IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY”, Indonesian Journal of Medical Laboratory Science and Technology, 1(2), pp. 58–64. doi: 10.33086/ijmlst.v1i2.1291.
Section
Articles
Ascorbic acid, papaya, iodimetry, UV-Vis spectrophotometry

References

Adib, M. (2018). Ekstrak Buah Pepaya (Carica Papaya L.) Meningkatkan Kadar Catalase dan Glutathione Hati Tikus yang Terpaparlead Acetate. Jurnal SainHealth, 2(1), 8–12.

Aghajanian, P., Hall, S., Wongworawat, M. D., & Mohan, S. (2015). The roles and mechanisms of actions of vitamin C in bone: new developments. Journal of Bone and Mineral Research, 30(11), 1945–1955.

Khoirunnisa, F., & Majid, A. (2014). Penentuan Kadar Vitamin C Dan Kadar Serat Kasar Yang Terkandung Dalam Buah-Buahan: Belimbing (Averhoa Carambola), Mangga (Mangifera Indica), Nanas (Ananas Comosus), Dan Pepaya (Carica Papaya). Jurnal Zarah, 2(1).

Munson, J. W. (1991). Analisis Farmasi Metode Modern. Parwa B. Diterjemahkan Oleh Harjana. Surabaya: Airlangga University Press.

Ngibad, K., & Herawati, D. (2019). Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible. Borneo Journal of Medical Laboratory Technology, 1(2), 77–81.

Ngibad, K., & Pradana, M. S. (2019). Effect of Starch and Sulfuric Acid on Determination of Vitamin C in Papaya Fruit Using Iodimetri. INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY, 1(1), 15–21.

Rahman, N., Ofika, M., & Said, I. (2015). Analisis Kadar Vitamin C Mangga Gadung (Mangifera sp) Dan Mangga Golek (Mangifera indica L) Berdasarkan Tingkat Kematangan Dengan Menggunakan Metode Iodimetri. Jurnal Akademika Kimia, 4(1), 33–37.

Suketi, K., Poerwanto, R., Sujiprihati, S., & Widodo, W. D. (2010). Karakter fisik dan kimia buah pepaya pada stadia kematangan berbeda. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 38(1).

Tahir, M., Kusuma, A. T., & Ekawati, E. (2018). Analysis of Lycopene and Vitamin C Levels of Pomelo Citrus Fruit (Citrus maxima (Burm) Merr) Red n White Varieties From South Sulawesi. JCPS (Journal of Current Pharmaceutical Sciences), 2(1), 125–130.

Winarno, F. G. (1997). Food Chemistry and Nutrition. Jakarta: Gramedia.

Nosy Maria Riscahyani, Department of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Indonesia

Evy Ratnasari Ekawati, Department of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Indonesia

Khoirul Ngibad, Department of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Indonesia