UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK KULIT NANAS (Ananas comosus. L) TERHADAP PERTUMBUHAN Ralstonia solanacearum

Abstract

This study aims to determine the effectiveness of antibacterial pineapple peel extract (Ananas
comosus. L) on Ralstonia solanacearum growth. Pineapple peel extract (Ananas comosus.L) contains
antibacterial compounds of bromelin and tannin enzymes that are able to inhibit the growth of plantcausing

bacteria, Ralstonia solanacearum. Ralstonia solanacearum bacteria will be given 5
treatments with pineapple peel extract 10%, 30%, 60%, 100% and positive control Streptomicin
sulfate 0.01%, each repeated 5 times. The test was performed by adding 100μl of Ralstonia
solanacearum suspension which was diluted into 15ml Tetrazolium chloride agar medium which was
given 100 ml of pineapple peel extract. The results showed that the treatment of pineapple peel extract
had a significant effect on the growth of Ralstonia solanacearum. The average number of treated
bacteria 60% (4x107 CFU), 100% (8x106 CFU) and Streptomicin sulfate 0.01% (4x106 CFU) was
significant (P <0.01) higher than the number of bacteria treated by concentration 10% (1.79x108)
and 30% (1.27x108). Consentration 100% pineapple peel extract (Ananas comosus.L) is the most
effective in inhibiting the growth of Ralstonia solanacearum. Thus it can be concluded that pineapple
peel extract (Ananas comosus.L) is effective as an antibacterial Ralstonia solanacearum.