COMPARATIVE STUDY OF IN-VITRO TOXICITY OF RAW HONEY AND FERMENTED HONEY USING THE BSLT (BRINE SHRIMP LETHALITY TEST) METHOD

##plugins.themes.academic_pro.article.main##

Devyani Wulansari
devyana dyah wulandari
Affina Krisdayanti

Abstract

Honey is an alternative product used in traditional medicine because of concerns, honey is a sweet liquid made by bees using flower nectar. Raw honey has low water content and high sugar content, when the water content in honey is high it makes honey easy to ferment. The samples used were raw honey and fermented honey. Raw and fermented honey contains phenolic compounds that can act as anticancer candidates by testing anticancer compounds in the form of toxicity measurements using the BSLT method. The honey sample yielded a toxicity of 59.75 μg/mL. While the fermented honey sample obtained an LC50 value of 3.28 μg/mL, which means the LC50 value was 30 ppm, indicating that the fermented honey sample was included in the very toxic category. Toxicity test on raw honey samples showed the LC50 value was in the toxic range. In the toxicity test, the fermented honey sample showed an LC50 value of 30 ppm, which means that the fermented honey has a very toxic toxicity value. There are differences in LC50 values in the second sample. However, statistically, there is no significant difference between raw honey and fermented honey

##plugins.themes.academic_pro.article.details##

How to Cite
Wulansari, D., dyah wulandari, devyana ., & Krisdayanti, A. . (2022). COMPARATIVE STUDY OF IN-VITRO TOXICITY OF RAW HONEY AND FERMENTED HONEY USING THE BSLT (BRINE SHRIMP LETHALITY TEST) METHOD. Medical Technology and Public Health Journal, 6(2), 148–156. https://doi.org/10.33086/mtphj.v6i2.3477

References

  1. Lobo V, Patil A, Phatak A., & Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. 2010. Pharmacognosy reviews, 4(8),118–126. https://doi.org/ 10.4103/0973- 7847.70902 DOI: https://doi.org/10.4103/0973-7847.70902
  2. Werdhasari A, Peran Antioksidan Bagi Kesehatan.2014. Jurnal Biotek Medisiana Indonesia, pp. 59-68.
  3. Purnomo DW, Didi U and Hadiah JT,. Prediksi Lebar Tajuk Pohon Dominan pada Pertanaman Jati Asal Kebun Benih Klon di Kesatuan Pemangkuan Hutan Ngawi, Jawa Timur, 2018. Jurnal Ilmu Kehutanan pp. 61–73. DOI: https://doi.org/10.22146/jik.34121
  4. Biologi J, et al. Uji kualitas madu pada beberapa wilayah budidaya lebah madu di kabupaten pati. 2017 Volume 6 (2).
  5. Saputri DS and Putri YE. Aktivitas Antioksidan Madu Hutan Di Beberapa Kecamatan Di Kabupaten Sumbawa Besar. Jurnal Tambora. 2017.Volume 2.
  6. Abubakar MB, Abdullah WZ, Sulaiman SA, & Suen AB. A review of molecular mechanisms of the anti-leukemic effects of phenolic compounds in honey.2012. International journal of molecular sciences, 13(11), 15054–15073. https://doi.org/10.3390/ijms131115054 DOI: https://doi.org/10.3390/ijms131115054
  7. Jacqueline, Deep Roots at Home.2021. [Online] [Diakses March 2022].
  8. Harborne JB. Metode Fitokimia: Penuntun Cara Modern Menganalisa. Tumbuhan.1987.Bandung: ITB Press. Harbone,
  9. Waghulde S, Kale MK, Patil V. Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants. 2019. Proceedings, 41, 47. https://doi.org/10.3390/ecsoc-23-06703 DOI: https://doi.org/10.3390/ecsoc-23-06703
  10. Septiana E and Simanjuntak P. Ekstrak Etanol Daun Dan Kulit Batang Bintangur (Calophyllum rigidum Miq .) Toxicity and in vitro antioxidant activity of ethanolic extracts of bintangur ( Calophyllum rigidum Miq .) leaf and stem bar. 2017. 10(1), pp. 10–16. DOI: https://doi.org/10.22435/toi.v10i1.7563.10-16
  11. Tyastirin E. & Hidayati I.. Statistika Parametrik Untuk Penelitian Kesehatan. 2017. 1st ed. Surabaya: Program Studi Arsitektur UIN Sunan Ampel.
  12. Martiningsih NW. Skrining Awal Ekstrak Etil Asetat Spons Leucetta sp . Sebagai Antikanker dengan Metode Brine Shrimp Lethality Test ( BSLT ). 2013. Seminar Nasional FMIPA UNDIKSHA III, pp. 382–386.
  13. Dinar SS, and Yolli EP. Aktivitas Antioksidan Madu Hutan Di Beberapa Kecamatan Di Kabupaten Sumbawa Besar. 2017. Vol. 2 No. 3: Edisi 5 DOI: https://doi.org/10.36761/jt.v2i3.170 DOI: https://doi.org/10.36761/jt.v2i3.170
  14. Wulandari, DD. Kualitas Madu (Keasaman, Kadar Air, Dan Kadar Gula Pereduksi) Berdasarkan Perbedaan Suhu Penyimpanan. 2017. Jurnal Kimia Riset, Volume 2 No. 1. DOI: https://doi.org/10.20473/jkr.v2i1.3768
  15. Wulansari, DD. Madu Sebagai Terapi Komplementer. 2018 Yogyakarta: Graha Ilmu.
  16. Dewi SR, Salim H, and Karim D. Efek Pemberian Perasan Bawang Putih Lanang (Allium Sativum L.) Terhadap Daya Hambat Pertumbuhan Candida albicans, Streptococcus mutans dan Propionibacterium acnes, 2021. Media Farmasi, 16(1), p. 124. DOI: https://doi.org/10.32382/mf.v16i1.1415 DOI: https://doi.org/10.32382/mf.v16i1.1415
  17. Rumaseuw SE, et al. Review: Perbandingan Uji Keamanan antara Bawang Putih Lanang Dan Bawang Hitam Lanang. 2014
  18. Ningdyah WA, Alimuddin HA and Jayuska A. Uji Toksisitas Dengan Metode Bslt (Brine Shrimp Lethality Test) Terhadap Hasil Fraksinasi Ekstrak Kulit Buah Tampoi (Baccaurea macrocarpa). 2015. 4(1), pp. 75–83.
  19. Andini A, et al. Pengaruh Penggunaan Jenis Pelarut Dalam Uji Sitotoksistas Metode Brine Shrimp Lethality Test (Bslt) Pada Wound Dressing Kolagen-Kitosan. 2021 DOI: https://doi.org/10.15575/ak.v8i1.10277
  20. Sumarlin LO, Muawanah A, and Wardhani P. Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia (Anticancer and Antioxidant Activity of Honey in the Market Local Indonesia). 2014. Jurnal Ilmu Pertanian Indonesia (JIPI)
  21. Zulfiah, et al. Uji Toksisitas Ekstrak Rimpang Temu Hitam (Curcuma Aeruginosa Roxb.) Terhadap Larva Udang (Artemia Salina Leach) Dengan Metode Brine Shrimp Lethality Test (BSLT). 2020. Volume VI, No.1. Jurnal Farmasi Sandi Karsa (JFS) DOI: https://doi.org/10.36060/jfs.v6i1.67