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Yudhiakuari Sincihu


Microplastics have been identified as food chain contaminants. Most plastic polymers are polyethylene. Microplastics in the gastrointestinal tract will undergo persorption through the cell gap at the end of the intestinal villi into the blood circulation to be distributed throughout the tissue. It is suspected that microplastics will trigger chronic inflammation. The aim of the study was to analyze the impact of oral exposure to polyethylene microplastics on the blood leukocyte cell count. Pure experimental research with posttest only control group design. Random allocation was used to divide 30 Rattus norvegicus wistar strains into each group of the same number. There were 4 exposure groups with a dose of D1=0.0375mg, D2=0.075mg, D3=0.15mg, D4=0.6mg of microplastic particles per day through an oral probe and 1 control group that was not given microplastics. Exposure is given for 90 days. One-way ANOVA test was used to analyze the difference in values ​​between groups. The results of this study show an increasing trend in the mean of leukocyte biomarkers, neutrophil and basophil counts in the exposure group (1,2,3,4) compared to the control (0). Statistical tests showed significant values ​​for leukocyte biomarkers (P= .048) and basophils (P= .040), where more exposure doses will cause an increase in the level of these biomarkers in the blood. Oral exposure to polyethylene microplastics had an effect on leukocytosis in the blood of Rattus norvegicus wistar strain.


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Counting blood cell, chronic infection, leukocytosis, microplastics, polyethylene


Prokić MD, Radovanonić TB, Gavarić JP, Faggio C. Ecotoxicological effects of microplastics: Examination of biomarkers, current state and future perspectives. TrAC. 2019;111: 37–46.

Jambeck JR, Geyer R, Wilcox C, Siegler TR, Perryman M, Andrady A, Narayan R, Law KL. Plastic waste inputs from land into the ocean. Sci J. 2015; 347(6223): 768–772.

Widianarko B, Hantoro I. Mikroplastik dalam Seafood dari Pantai Utara Jawa. Semarang: Universitas Katolik Soegijapranata; 2018.

Wang YL, Lee YH, Chiu IJ, Lin YF, Chiu HW. Potent impact of plastic nanomaterials and micromaterials on the food chain and human health. Int J Molecular Sci. 2020; 21(5):1-8.

Campanale C, Massarelli C, Savino I, Locaputo V, Uricchio VF. A detailed review study on potential effects of microplastics and additives of concern on human health. Int J Environ Res Pub Heal. 2020; 17(4):1212-1219.

Wright SL, Kelly FJ. Plastic and human health: a micro issue?. Environ Sci Tech. 2017; 51(12): 6634–6647.

Deng Y, Zhang Y, Lemos B, Ren H. Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure. Sci.Rep. 2017; 7(46687): 1-10.

Doda DVD, Polii H, Marunduh S, Sapulete IM. Fisiologi sistem hematologi. Manado: Universitas Sam Ratulangi; 2020.

Hoffbrand AV, Pettit JE, Moss PAH. Hematologi. Edisi 4. Jakarta: ECG; 2014.

Monteleone A, Schary WM, Fath A, Wenzel F. Validation of an extraction method for microplastics from human materials. Clin Hemorh Microcir. 2019; 73(8): 1-15.

Schwabl P, Köppel S, Königshofer P, Bucsics T, Trauner M, Reiberger T, Liebmann B. Detection of various microplastics in human stool: a prospective case series. Ann Intern Med. 2019; 171(7): 453-457.

Ragusa A, Svelato A, Santacroce C, Catalano P, Notarstefano V, Carnevali O, Papa F, Rongioletti MCA, Baiocco F, Draghi S, D'Amore E, Rinaldo D, Matta M, Giorgini E. Plasticenta: first evidence of microplastics in human placenta.

Environ Int. 2021; 146(106274): 1-8.

Hwang J, Choi D, Han S, Jung SY, Choi J, Homong J. Potential toxicity of polystyrene microplastic particles. Sci Report. 2020; 10(7391): 1-12.

Kole PJ, Löhr AJ, Belleghem FGAJV, Ragas AMJ. Wear and tear of tyres: a stealthy source of microplastics in the environment. Int J Environ Res Public Health. 2017; 14(10): 1-31.

Dehaut A, Cassone AL, Frere L, Hermabessiere L, Himber C, Rinnert E, Riviere G, Lambert C, Soudant P, Huvet A, Duflos G, Paul-Pont I. Microplastics in seafood: benchmark protocol for their extraction and characterization. Environ Poll. 2016; 215: 223–233.

Prata JC, da Costa JP, Lopes I, Duarte AC, Rocha-Santos T. Environmental exposure to microplastics: an overview on possible human health effects. Sci Tot Environ. 2019; 702(134455): 1-31.

Smith M, Love DC, Rochman CM, Neff RA. Microplastics in seafood and the implications for human health. Cur Environ Heal Reports. 2018; 5: 375-386.

Deng Y, Zhang Y, Qiao R, Bonilla MM, Yang X, Ren H, Lemos B. Evidence that microplastics aggravate the toxicity of organophosphorus flame retardants in Mice (Mus Musculus). J Hazard Mat. 2018; Jun(6): 1-28.

Othman A, Hasan HA, Muhamad MH, Ismail NI, Abdullah SRS. Microbial degradation of microplastics by enzymatic processes: a review. Environ Chem Letters. 2021; 19(4): 3057–3073.

Kantari C, Pederzoli-Ribeil M, Witko-Sarsat V. The role of neutrophils and monocytes in innate immunity. Tren Innate Immun. 2008; 15: 118–146.

Amulic B, Cazalet C, Hayes GL, Metzler KD, Zychlinsky A. Neutrophil function: from mechanisms to disease. Ann Rev Immun. 2012; 30: 459-489.