Fibrinolytic and coagulation activities of crude extracellular preparation from Bacillus cereus group strain SF-4
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Muhammad Ardi Afriansyah
https://orcid.org/0000-0002-5368-6080
Sri Sinto Dewi
https://orcid.org/0009-0005-7042-4110
Carissa Erlinda Rahma Putri
https://orcid.org/0009-0003-2584-7056
Medhat Ahmed Abu-Tahon
https://orcid.org/0000-0002-2215-3660
Stalis Norma Ethica
https://orcid.org/0000-0002-0853-0423
Ana Hidayati Mukaromah
https://orcid.org/0000-0001-8492-4961
Hayatun Fuad
https://orcid.org/0009-0009-3954-026X
Abstract
Fibrinolytic enzymes have attracted considerable attention as potential alternatives to conventional thrombolytic agents because they degrade fibrin, a major structural component of blood clots. Fermented seafood products are promising sources of protease-producing microorganisms; however, fermented bamboo clam (Solen sp.) rusip remains underexplored as a source of bacteria with fibrinolytic potential. This study aimed to isolate and screen protease-producing bacteria from this product and to evaluate the fibrinolytic, thrombolytic, and whole-blood clotting-time effects of a crude extracellular preparation from the selected isolate. Eight bacterial isolates were recovered, and isolate SF-4 exhibited the highest proteolytic activity, was selected for further evaluation. Based on 16S rRNA gene sequencing, SF-4 was assigned to the Bacillus cereus group. The crude extracellular preparation was obtained after cultivation at 37 °C for 72 h and was assessed using fibrin agar, a blood clot-lysis assay, and a whole-blood clotting-time assay. The preparation showed a fibrinolytic index of 1.33 ± 0.17, produced 13.51 ± 2.72% clot lysis, and prolonged whole-blood clotting time to 9.30 ± 0.26 min (mean ± SD, n = 3). Differences among treatments were performed using one-way ANOVA. These findings provide preliminary functional evidence of fibrinolysis-related activity in a bacterium from fermented bamboo clam rusip. However, toxin-gene screening, quantitative haemolysis testing, activity normalisation, and pathway-specific coagulation assays were not performed.
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Copyright (c) 2026 Muhammad Ardi Afriansyah, Sri Sinto Dewi, Carissa Erlinda Rahma Putri, Medhat Ahmed Abu-Tahon, Stalis Norma Ethica, Ana Hidayati Mukaromah, Hayatun Fuad
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