The Effect of Honey Administration on The Histopathology of The Duodenum of Wistar Rats as a Inhibition of The Toxic Effects of Borax (Sodium tetraborate)
Article Sidebar
Main Article Content
Abstract
Background: Borax can result in oxidative stress and cause the onset of gastrointestinal ulcerations that will dampen the duodenal villi to become shorter and can even disappear. Cell damage due to oxidative stress can be prevented by administering antioxidants. Antioxidants will inhibit the onset of chain reactions in the formation of free radicals by complementing the existing electron deficiency. Honey is one of the natural ingredients that is rich in antioxidants and part of thibbun Nabawi as one of the inhibitors of the toxic effects of borax.
Objective : The purpose of this study is to to analyze the effect of honey administration on the histopathological description of submucosa and epithelial mucosa of duodenal wistar rats as an inhibitor of the toxic effects of borax (Sodium tetraborate).
Methods: This study used the true experimental method with Post Test Only Control Group Design using 25 mice which will be divided into five groups, namely K (negative control), P (positive control), M1 (borax and honey dose 1), M2 (borax and honey dose 2), M3 (borax and honey dose 3). This study was conducted for 22 days then. Took the duodenal organ on all samples and then made histological preparations with HE staining. Observations were made with an Olympus microscope to see submucosal edema and damage to the epithelium of the duodenal mucosa.
Results: The results of the study found that borax 26 mg / head / day had an influence on the histopathological picture of duodenal borax. In addition, the administration of honey dose 75 mg / Kg BB provides a toxic inhibitory effect of borax in the duodenum best among other treatment groups. However, statistically there was no effect of honey administration on the histopathological picture of the duodenum of wistar rats as an inhibitor of the toxic effect of borax (Sodium Tetraborate) with p˃0.05.
Conclusion: Statistically it can be concluded that there is no effect of the administration of honey on the histopathology picture of the duodenum of wistar rats as an inhibitor of the toxic effects of borax (Sodium tetraborate).
Downloads
Article Details
Copyright (c) 2023 Dian Noviana, Mustika Chasanatusy Syarifah, Retna Gumilang, Tri Wahyuni Bintarti
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Amalia, R., Wurlina., & Hestianah, E. P. 2017. Efek Pemberian Vitamin C Dan Vitamin E Terhadap Gambaran Histopatologi Duodenum Mencit Yang Dipapar Boraks. Jurnal Veterina Medika, 10(1): 23 – 30.
Anggraeni, D. 2018. Pengaruh Terapi Preventif Madu Hutan Sumbawa terhadap Kadar Malondialdehida (MDA) dan Histopatologi Duodenum Tikus (Rattus novergicus) yang Diinduksi Plumbum Asetat. Skripsi. FKH UB. Program Studi Kedokteran Hewan.
BPOM RI. 2021. Intensifikasi Pengawasan Pangan Selama Bulan Ramadhan Hingga Menjelang Idul Fitri. Sistem Keamanan Pangan Terpadu oleh Badan Pengawas Obat dan Makanan RI.
Cahyani, DI. 2017. Pengaruh Penambahan Teh Hijau terhadap Aktivitas Antioksidan dan Kadar Protein Minuman Fungsional Susu Kedelai dan Madu. Skripsi. FK Diponegoro. Program Studi Ilmu Gizi.
Elziyad, MT. dan Widjaja, NMR. dan Plumeriastuti, H. 2013. Pengaruh Boraks terhadap Gambaran Histopatologi Duodenum Tikus Putih (Rattus novergicus). Julnal Veterina Medika, 6(2):133 – 138.
Fatahilah, M. 2016. Klinik Pengobatan Thibbun Nabawi di Kota Pontianak. Jurnal Online Mahasiswa, 4(2):108-118.
Pratama, A. Y. 2016. Pengaruh Terapi Preventif Madu Hutan Riau terhadap Kadar MDA dan Kadar ALP pada Serum Hewan Model Tikus yang Diinduksi CCL4. Skripsi. FKH UB. Program Studi Kedokteran Hewan.
Pujaswarini, N. M. H., Berata, K., & Setiasih, N. L. E. 2019. Ekstrak Daun Kelor Memulihkan Perubahan Histopatologi dan Morfometri Duodenum Tikus Setelah Aktivitas Fisik Berlebih. Jurnal Indonesia Medicus Veterinus, 8(6): 739 – 749.
Purnama, M. T. E., Widjaja, N. M. R., & Plumeriatusi, H. 2013. Pengaruh Boraks terhadap Gambaran Histopatologi Duodenum Tikus Putih (Rattus novergicus). Skripsi. FKH UNAIR. Program Studi S1 Pendidikan Dokter Hewan.
Sirait, R.C., Tjahjono, K., & Setyawati, A. N. 2016. Pengaruh Pemberian Ekstrak Jintan Hitam (Nigella sativa) terhadap Kadar MDA Serum tikus Sparague Dawley Setelah Diberikan Paparan Asap Rokok. Jurnal Kedokteran Diponegoro, 5(4): 1603 – 1612. DOI: https://doi.org/10.35790/ebm.4.1.2016.10866