The Effect of Mercury Exposure to Escherichia Coli Bacteria Resistant to Mercury and Escerichia Coli Esbl in Vitro
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Abstract
Background: The level of pollution in Indonesia is still very high, consist of water pollution, air pollution and soil pollution. Mercury is one of the heavy metals that pollutes the waters of the sea, while Escherichia coli is exposed to mercury will try to defend itself by doing mercury detoxification so that it can live in an environment that contains mercury. Escherichia coli that tries to defend itself from mercury exposure in the environment will experience a change in its genes into mercury resistant Escherichia coli. In plasmids or transposons, it might also stimulate the formation of resistance genes for some antibiotics, include producing the ESBL enzyme, so that it can convert non ESBL Escherichia coli into ESBL Escherichia coli. Objective: This study aims to prove that the repeated exposure of mercury will change non ESBL-mercury sensitive Escherichia coli into ESBL- mercury resistant Escherichia coli. Method: This was an experimental study with 27 non-ESBL Escherichia coli isolates as identified from Phoenix. Non-ESBL Escherichia coli clinical isolates were tested by giving exposure to HgCl2 with concentrations of 0.02 ppm, 0.10 ppm, 0.20 ppm for 1-14 days until mercury resistant Escherichia coli was formed, and then ESBL screening was tested by giving Cefotaxime exposure to them.
Results: On the first day of mercury exposure, there were 9 isolates of 0.02 ppm HgCl2 resistant Escherichia coli, 9 isolates of 0.10 ppm HgCl2 resistant Escherichia coli, 9 isolates of 0.20 ppm HgCl2 resistant Escherichia coli. Furthermore, this Escherichia coli isolate was exposed to Cefotaxim as ESBL screening. The final results of post-exposure HgCl2 0.02 ppm was obtained 3 (33.3%) isolates were still sensitive to Cefotaxime and 6 (66.7%) isolates that were resistant to Cefotaxime. The final results of post-exposure HgCl2 0.10 ppm was obtained all 9 (100%) isolates that were resistant to Cefotaxime. The final results of post-exposure HgCl2 0.20 ppm obtained 2 (22.2%) isolates were still sensitive to Cefotaxime and 7 (77.8%) isolate were resistant to Cefotaxime. Conclusion: Escherichia coli in urine had the phenotive change into mercury resistant Escherichia coli. Mercury exposure of 0.02 ppm, 0.10 ppm, 0.20 ppm for 1 day in vitro on isolates of non ESBL-mercury resistant Escherichia coli caused changes in 22 isolates of Escherichia coli in urine
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References
Barkay, T., S.M. Miller, A.O. Summers. 2003. Bacterial Mercury Resistance from Atoms to Ecosystems. FEMS Microbiol. Rev. 27:355-384.
Boyd, Eric S., Tamar Barkay. 2012. The mercury resistance operon: from an origin in a geothermal environment to an efficient detoxification machine. Frontiers in Microbiology. Published October 2012. Accessed from https://www.frontiersin.org/articles/10.3389/fmicb.2012.00349/full.
Brown, N., Shih, Y., Leang,C., Glendinning, K., Hobman, J & Wilson, J. 2002. Mercury transport and resistance Biometals, Internasional Biometals Symposium
Cantón R, Coque TM, 2006, The CTX-M beta-lactamase pandemic, Servicio de Microbiología, Hospital Ramón y Cajal, 28034-Madrid, Spain.
Chojnacka K , 2010, Biosorption and bioaccumulation--the prospects for practical applications, 36(3):299-307. doi: 10.1016/j.envint.2009.12.001. Epub 2010 Jan 6, Diakses tgl 20 Januari 2017.
Hinonaung Hamonangan Jefry, Bodhi Widdhi, KepelI Billy, 2013, Identifikasi Bakteri Resisten Merkuri Pada Individudi Daerah Pesisir Pantai Di Desa Budokecamatan Wori, Bagian Kimia Fakultas Kedokteran Universitas Sam Ratulangi.
Palar, H. 2008. Pencemaran dan Toksikologi Logam Berat. Rinekacipta : Bandung. Republika.
Ririn,Ayu Dwi. 2013. Isolasi Dan Uji Resistensi Antibiotik Bakteri. Resistensi Merkuri (Hg) Dari Kawasan Pantai. Losari Makassar, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Hasanuddin Makasar.
Saraswati, D., Fahruddin. 2012. Uji Resistensi Antibiotik pada Bakteri Resisten Merkuri (Hg) yang di Isolasi dari Kawasan Pantai Losari Makassar Test of Antibiotic Resistance in Bacteria Resistant Mercury (Hg) in Isolation from Makassar Losari Region, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Hasanuddin
Talan DA, Takhar SS, Krishnadasan A, Abrahamian FM, 2016. Fluoroquinolone-Resistant and Extended-Spectrum β-Lactamase–Producing Escherichia coli Infections in Patients with Pyelonephritis, United States. Emerging Infectious Diseases 22(9); 1594-604
Walker KE, Mahon CR, Lehman D, Manuselis G, 2015. Enterobacteriaceae. InText book of diagnostic microbiology 5th ed. pp 420-54
Widowati W., Astiana S. dan Raymond J.R., 2008, Efek Toksik Logam, Pencegahan dan Penanggulangan Pencemaran. Penerbit ANDI, Yogyakarta