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PRECIOUS EBISINTEI Hotimah Masdan Salim

Abstract

Introduction: Coconut milk is a very nutritional milk or drink. The processor of coconut into milk using various forms has been A serious risk and health hazard.
Objective: This study aims to describe or evaluate the rate of microbial activity in coconut milk when stored.
Methods: The coconut milk was extracted from the coconut meat using an aseptic technique.
Results: The initial PH value was 6.10, it was observed that fermentation started from the 12th hour, and at the 24th hour, the PH of the coconut milk was 4.48. After 48 hours, the pH was 4.20 and at the 72nd hour time, the pH value was 3.99. It was also observed that the 24 hours stored coconut milk sample has the highest Bacterial growth rate of 4.12 × 102 and a fungal growth rate of 1.2 × 102.
Conclusions: Biochemical test carried out on the bacterial isolate showed Bacillus spp, Staphylococcus aureus, and Bifidobacterium bifidum were observed to be present at 24 hours and 48 hours of the coconut milk sample and at the 72nd hours of the coconut milk sample Pseudomonas spp, Lactobacillus spp, and Aeromonas caviae were observed to be present. A drop of pH value to 3.99 at the 72nd hour was observed due to the activity of Lactobacillus spp

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How to Cite
EBISINTEI, P., & Salim, H. M. (2024). Evaluation of The Rate of Microbial Activity in Coconut (Cocos Nucifera L.) Milk When Stored at Different Time Intervals. International Islamic Medical Journal, 6(2), 40–53. https://doi.org/10.33086/iimj.v6i2.6480
Section
Articles
Coconut milk, Microbial activity, Fermentation.

References

Alfred, K. O. (2012). Development of Cheese product from coconut milk; Department of Biotechnology, Faculty of Bioscience, College of Science and Technology Kumasi, Ghana, 1(1).

Alyaqoubi, S., Abdullah, A., Samudi, M., Abdullah, N., Addai, Z. R., & Musa, K. H. (2015). Study of antioxidant activity and physicochemical properties of coconut milk (Pati santan) in Malaysia. Journal of Chemical and Pharmaceutical Research, 7(4), 967-973.

Asiri N. karunasiri, Mahendra Gunawardane, Chathuri M. Seunayake, Nimanthi Jayathilaka and Kapila N. Seneviratne. (2020). Antioxidant and Nutritional Properties of Domestic and Commercial Coconut milk preparation. International Journal of Food Science. https://dio.org/10.1155/2020/3489605

Benyagoub Elh., Ayat, M., Dahan, T. and Smahi, K. (2013). Level of control of the hygienic quality of camel milk (Camelus dromedarius) in south west Algeria and its impact on security. Peak Journal of Food Science and Technology 1(4): 53-60.

Che Man YB, Abdul Karim MIB, Teng CT. (1997). Extraction of coconut oil with Lactobacillus plantarum 1041 IAM, J. of American Oil Chemists Society, 74, 1115–1119.

Cheesbrough, M. (2005). District laboratory practice in tropical countries, part 2. Cambridge university press.

DebMandal, M., & Mandal, S. (2011). Coconut (Cocos nucifera L.: Arecaceae): in health promotion and disease prevention. Asian Pacific journal of tropical medicine, 4(3), 241-247

De Vuyst L, Leroy F (2004). Fermented food in the context of a healthy diet: How to produce novel functional food? Journal of Current Opinion in Clinical Nutrition and Medical Care 17:574-581.

Fawole, M. O., & Oso, B. A. (2004). Biochemical test. Laboratory manual of microbiology. Femi Ibirogba. (2019), Demand for Coconut has Increased by 500% (Business Agro). The Guardian Newspaper.

Fleetwood, J., Rahman, S., Holland, D., Millson, D., Thomson, L., & Poppy, G. (2019). As clean as they look? Food Hygiene inspection scores, microbiological contamination, and foodborne illness. Food Control, 96, 76–86. https://doi.org/10.1016/j.foodcont.2018.08.034

Hafidh, R. R., Abdulamir, A. S., Law, S. V., & Fatimah, A. B. (2010). The inhibition of human pathogens: Trichophyton rubrum and Trichoderma harzianum by a natural product. American Journal of Biochemistry and Biotechnology, 6(1), 40-46.

Jordana, J. (2000). Traditional foods: challenges facing the European food industry. Food Research International, 33(3-4), 147-152.

Mabesa, R. C., & del Rosario, R. R. (1979). Quality control in coconut milk processing, 2: microbial contaminants [study conducted in the Philippines]. Philippine Agriculturist.

Nurul, F.M, Noorlis, A, Nurul Ain, H. and Suwaibah, M. (2017). Enumeration of Escherichia coli from fresh Coconut milk in Negeri Sembilan: International Food Research Journal24(1): 445-458.

Omobayonle, A. (2020). Lactic acid bacteria fermentation of coconut milk and its effect on the nutritional, phytochemical, antibacterial and sensory properties of virgin coconut oil produced. African Journal of Biotechnology, 19(6), 362-366

Pamplona-Roger, G. (2007). Foods and their healing power; a guide to food science and diet therapy. Vol 2. Nexo Grafico, Spain. pp 325-328.

Rethinam, P. (2006). Asian and Pacific Coconut Community activities, achievements and future outlook. In ACIAR PROCEEDINGS (Vol. 125, p. 15). ACIAR; 1998.

Satheesh, N., & Prasad, N. (2013). Optimization of parameters for fermentative production of virgin coconut oil by Lactobacillus sp. Food Sci. Technol, 14, 312-317.

Sari, P. M., & Muhlisin, A. (2019). Staphylococcus aureus in traditional coconut milk drinks Tropical Health and Medical Research, 1(1), 33–38. https://doi.org/10.35916/thmr.v1i1.1

Timmen, H. and S. Patton (1989): Milk fat globules: fatty acid composition, size and in vivo regulation of fat liquidity. Lipids 23:685-689.

Thanuja T. T., G.S.Sreekala and Meenakumari.(2016). Isolation of microorganisms from the Fermented coconut milk and comparison of quality parameters of virgin coconut oil Recovered by induced fermentation. International Journal of Advanced Research (2016), Volume 4, Issue 6, 1927-1934.

Yoon, L. W., Ang. T. N., Ngoh, G.C., Chua, A. S. M. (2014). Fungal solid- state fermentation and Various methods of enhancement in Cellulase production, Biomass Bioenerg. 67, 319.

PRECIOUS EBISINTEI, Department of Biological Sciences, Faculty of Basic and Applied Sciences, University of Africa Toru Orua

Hotimah Masdan Salim, Department of Biochemistry and Biologimolecular, Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya