Kajian Poliploidi Ikan Sepat (Trichogaster trichopterus) pada Tiga Ketinggian Tempat Berbeda di Daerah Banyuwangi, Malang, dan Batu dengan Metode Penghitungan Nukleolus
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Abstract
Penelitian ini bertujuan untuk mengetahui pengaruh tiga tempat ketinggian yang berbeda di daerah Banyuwangi, Malang, dan Batu terhadap tingkat poliploidi ikan Trichogaster trichopterus. Poliploidi yang diamati adalah 3n dan 4n. Metode yang digunakan adalah analisis penghitungan nukleolus menggunakan teknik pewarnaan perak nitrat (AgNO3). Jaringan yang digunakan untuk menghitung jumlah nukleolus adalah sirip ekor ikan. Sampel menggunakan 30 ikan Trichogaster trichopterus di tiga tempat berbeda dengan 10 ikan berbeda dari Banyuwangi (99 mdpl), 10 ikan dari Malang (982 mdpl), dan 10 ikan dari Batu (1668 mdpl). Analisis data menggunakan uji Kruskal Wallis dan untuk mengetahui hubungan di setiap ketinggian digunakan uji Mann Whitney. Hasil penelitian ini menunjukkan bahwa ketinggian tempat berpengaruh terhadap tingkat poliploidi Trichogaster trichopterus. Ketinggian rendah tidak berbeda secara signifikan dari ketinggian sedang, tetapi ketinggian sedang secara signifikan berbeda dari ketinggian tinggi.
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Kundariati, M., Wulan, R. C., & Sudrajat, A. K. (2018). Kajian Poliploidi Ikan Sepat (Trichogaster trichopterus) pada Tiga Ketinggian Tempat Berbeda di Daerah Banyuwangi, Malang, dan Batu dengan Metode Penghitungan Nukleolus. Applied Technology and Computing Science Journal, 1(2), 76–85. https://doi.org/10.33086/atcsj.v1i2.855
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Articles
Ketinggian, Poliploidi, Trichogaster trichopterus
References
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[2] Carman, O., Oshiro, T., & Takashima, F. Estimation of effective condition for induction of triploidy in goldfish, Carassius auratus Linnaeu. Journal of the Tokyo University of Fisheries. 1991.
[3] Cires, E., Cuesta, C., Vargas, P., & Prieto, J. A. F. Unravelling the evolutionary history of the polyploid complex ranunculus parnassiifolius (ranunculaceae). Biological Journal of the Linnean Society. 2012; 107(3): 477–493. https://doi.org/10.1111/j.1095-8312.2012.01968.x
[4] Corebima, A. D. Genetika Mutasi dan Rekombinasi. Malang: FMIPA UM. 2000.
[5] Diallo, A. M. Coexistence and performance of diploid and polyploid Acacia senegal (L.) Willd: implications for adaptation and domestication in the Sahel. University of Copenhagen Rolighedsvej. 2015.
[6] Froese, R. (Rainer), & Pauly, D. (Daniel). FishBase 2000: concepts, design and data sources. 2000.
[7] Guertin, D. A., Trautmann, S., & McCollum, D. Cytokinesis in Eukaryotes. Microbiology and Molecular Biology Reviews. 2002; 66(2): 155–178. https://doi.org/10.1128/MMBR.66.2.155- 178.2002
[8] Hoar, W. S., Randall, D. J., & Brett, J. R. Fish Physiology (Vol. 8). London: ACADEMIC PRESS, INC. 1979. https://doi.org/10.1016/S1546-5098(08)60020-5
[9] Kadi, A. Manipulasi Poliploidi Untuk Memperoleh Jenis Baru Yang Unggul. Oseana. 2007; XXXII(4): 1–11.
[10] Kim, S., Lee, D., & Rayburn, A. L. Analysis of Active Nucleolus Organizing Regions in Polyploid Prairie Cordgrass (Spartina pectinata Link) by Silver Staining. Cytologia. 2015; 80(2): 249–258. https://doi.org/10.1508/cytologia.80.249
[11] Kottelat, M., Whitten, A., Kartikasari, S., & Wirjoatmodjo, S. Ikan Air Tawar Indonesia Bagian Barat dan Sulawesi. Jakarta: Periplus Edition (HK) Ltd. dan Proyek EMDI KMNLH. 1993.
[12] Leggatt, R. A., & Iwama, G. K. Occurrence of polyploidy in the fishes. Reviews in Fish Biology and Fisheries. 2003; 13(3): 237–246. Retrieved from https://doi.org/10.1023/B:RFBF.0000033049.00668.fe
[13] Liu, S. J. Distant hybridization leads to different ploidy fishes. Science China Life Sciences. 2010; 53(4): 416–425. https://doi.org/10.1007/s11427-010-0057-9
[14] Mable, B. K., Alexandrou, M. A., & Taylor, M. I. (2011). Genome duplication in amphibians and fish: an extend synthesis. Journal of Zoology. 2011; 284(7): 151–182. https://doi.org/10.1111/j.1469-7998.2011.00829.x
[15] Murjani, A. Budidaya beberapa varietas ikan sepat rawa (Trichogaster trichopterus Pall) dengan pemberian pakan komersial. Lampung: Fakultas Perikanan Universitas Lampung Mangkurat. 2010.
[16] Paule, J., Kolář, F., & Dobeš, C. Arctic-alpine and serpentine differentiation in polyploid Potentilla crantzii. Preslia. 2015; 87(2): 195–215.
[17] Phillips, R. B., Zajicek, K. D., Ihssen, P. E., & Johnson, O. Application of silver staining to the identification of triploid fish cells. Aquaculture. 1986; 54(4): 313–319. https://doi.org/10.1016/0044-8486(86)90275-9
[18] Piferrer, F., Beaumont, A., Falguière, J., Flajshans, M., Haffray, P., & Colombo, L. Polyploid fi sh and shell fi sh : Production , biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture. 2009; 293(3–4): 125–156. https://doi.org/10.1016/j.aquaculture.2009.04.036.
[19] Purwantara, S. Studi Temperatur Udara Terkini di Wilayah di Jawa Tengah dan DIY. Informasi. 2009; 37(2): 11–30.
[20] Ramsey, J., & Ramsey, T. S. (2014). Ecological studies of polyploidy in the 100 years following its discovery. Philosophical Transactions of the Royal Society B: Biological Sciences. 2014; 369: 15–19. https://doi.org/10.1098/rstb.2013.0352
[21] Risnandar, D. Pengaruh Umur Zigot pada Saat Kejutan Panas terhadap Keberhasilan Triploidisasi serta Kelangsungan Hidup Embrio dan Larva Ikan Jambal Siam (Pangasius hypopthalmus). Bogor: Institut Pertanian Bogor. 2001.
[22] Rottmann, R. W., Shireman, J. V., & Chapman, F. A. Induction and Verification of Triploidy in Fish. SRAC Publication. 1991; (427): 1–2. Retrieved from https://srac.tamu.edu/serveFactSheet/90
[23] Schinkel, C. C. F., Kirchheimer, B., Dellinger, A. S., Klatt, S., Winkler, M., Dullinger, S., & Hörandl, E. Correlations of polyploidy and apomixis with elevation and associated environmental gradients in an alpine plant. AoB Plants. 2016; 8: plw064. https://doi.org/10.1093/aobpla/plw064 [24] Song, C., Liu, S. J., Xiao, J., He, W. G., Zhou, Y., Qin, Q. B., & Liu, Y. Polyploid organisms. Science China Life Sciences. 2012; 55(4): 301–311. https://doi.org/10.1007/s11427-012-4310-2 [25] Soto-Trejo, F., Palomino, G., Villaseñor, J. L., & Crawford, D. J. (2013). Polyploidy in Asteraceae of the xerophytic scrub of the Ecological Reserve of the Pedregal of San Angel, Mexico City. Botanical Journal of the Linnean Society. 2013; 173(2): 211–229. https://doi.org/10.1111/boj.12080.
[26] Taylor, E. W. The Mechanism of Colchicine Inhibition of Mitosis. The Journal of Cell Biology. 1965; 25: 145–160. https://doi.org/10.1083/jcb.25.1.145.
[27] Thorgaard, G. H. Chromosome Set Manipulation and Sex Control in Fish. Fish Physiology. 1983; 9: 405–434. https://doi.org/10.1016/S1546-5098(08)60308-8.
[28] Thorgaard, G. H., Jazwin, M. E., & Stier, A. R. Polyploidy Induced by Heat Shock in Rainbow Trout. Transactions of the American Fisheries Society. 1981; 110(4): 546–550. https://doi.org/10.1577/1548-8659(1963)92.
[29] Tomasello, S. Polyploidy and high mountain environments: Evolutionary processes at different scales in the subtribe Leucanthemopsidinae (Compositae, Anthemideae). Universität Regensburg. 2014.
[30] Warianto, C. Mutasi. Surabaya: Publish. 2011.
[31] Wei, R., Ree, R. H., Sundue, M. A., & Zhang, X.-C. Polyploidy and elevation contribute to opposing latitudinal gradients in diversification and species richness in lady ferns (Athyriaceae). BioRxiv. 2018; 4. https://doi.org/10.1101/351080.
[32] World Bank. Integrating Freshwater Biodiversity Conservation with Development: Some Emerging Lessons. 1998.
[33] Xie-Kui, C., Cheng-Qi, A., Zhang, Q., Chen, L. T., & Liu, J. Q. Diploid and tetraploid distribution of allium przewalskianum regel. (liliaceae) in the qinghai-tibetan plateau and adjacent regions. Caryologia. 2008; 61(2): 192–200. https://doi.org/10.1080/00087114.2008.10589629