https://journal2.unusa.ac.id/index.php/IJMLST/issue/feedIndonesian Journal of Medical Laboratory Science and Technology2024-03-15T06:04:10+00:00Maharani Pertiwi Koentjoroijmlst@unusa.ac.idOpen Journal Systems<p><strong>Indonesian Journal of Medical Laboratory Science and Technology</strong> (IJMLST) is a double-blind peer-reviewed open access journal, which is issued bi-annual<strong> (April and October)</strong> reports on medical laboratory science dan medical laboratory technology. IJMLST published by <strong>Universitas Nahdlatul Ulama Surabaya</strong>, Indonesia. This includes reports from the following fields: <strong>hematology, clinical chemistry, immunoserology, microbiology, parasitology, cyto-histotechnology, clinical toxicology, and molecular biology</strong>. The submission of practical information for medical workforce in medical laboratory technology, development of innovative clinical and laboratory test methodology is welcomed. IJMLST is registered to International Standard Serial Number (ISSN) with p-ISSN: <a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&1556588519&1&&2019" target="_blank" rel="noopener">2684-6748</a> and e-ISSN: <a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&1554795870&1&&2019" target="_blank" rel="noopener">2656-9825</a>. This journal is also registered to CrossRef system with a DOI <a href="https://search.crossref.org/?q=Indonesian+Journal+of+Medical+Laboratory+Science+and+Tehnology+%28IJMLST%29" target="_blank" rel="noopener">10.33086/ijmlst</a>. IJMLST publishes only English-language articles. IJMLST is currently accredited on the Science and Technology Index with the predicate of <a href="https://sinta.ristekbrin.go.id/journals/detail?id=6922">Sinta 2</a> , <a href="https://doaj.org/toc/2656-9825?source=%7B%22query%22%3A%7B%22bool%22%3A%7B%22must%22%3A%5B%7B%22terms%22%3A%7B%22index.issn.exact%22%3A%5B%222684-6748%22%2C%222656-9825%22%5D%7D%7D%5D%7D%7D%2C%22size%22%3A100%2C%22sort%22%3A%5B%7B%22created_date%22%3A%7B%22order%22%3A%22desc%22%7D%7D%5D%2C%22_source%22%3A%7B%7D%2C%22track_total_hits%22%3Atrue%7D" target="_blank" rel="noopener">DOAJ </a>and <a href="https://suggestor.step.scopus.com/progressTracker/index.cfm?trackingID=D0F7F675E2BD6C93" target="_blank" rel="noopener">Scopus</a>.</p>https://journal2.unusa.ac.id/index.php/IJMLST/article/view/4549Screening of Haliclona sp. symbiont bacteria that have the potential as MDR (Multidrug-Resistant) antibacterial from Tanjung Tiram Beach2024-03-15T06:04:10+00:00Sri Anggarini Rasyidanggarini.09@gmail.comSugireng Sugirengsugireng92@gmail.comAsni Ramayana Tinaasni.tina001@gmail.com<p>Sponge <em>Haliclona</em> sp. is a type of sponge living in marine waters and is found in many areas of coral reef ecosystems. This study aimed to determine the potential for sponge <em>Haliclona </em>sp. obtained from Tanjung Tiram waters, Konawe, as a source of bioactive compounds of MDR (Multidrug-Resistant) antibacterial. The isolation method of <em>Haliclona</em> sp. symbiont was carried out by pouring and purification of <em>Haliclona </em>sp. symbiont using the scratch method. The purification results obtained 12 isolates of symbiont bacteria that were successfully isolated. Then the bacterial isolate was tested for its antibacterial ability against MRSA and ESBL <em>Escherichia coli</em> bacteria qualitatively using the agar diffusion method. Bacteria that have strong inhibitory activity are identified by morphological and biochemical tests. The results of the qualitative screening test produced four bacterial isolates that have inhibitory activity against MRSA (<em>Methicilin Resistant Staphylococcus aureus</em>) pathogenic bacteria with the isolate codes H3 and H8 belonging to the strong category while H4 and H10 belonging to the medium categoy. Additionally, two bacterial isolates that have inhibitory activity against ESBL <em>Escherichia coli</em> pathogenic bacteria with the isolate codes H3 and H8 belonging to the strong category. Based on morphological observations and biochemical tests, the H3 bacterial isolate was identified as the genus <em>Corynebacterium</em> spp. and the H8 bacterial isolate was identified as the genus <em>Micrococcus</em> spp. In conclusion, <em>Haliclona</em> sp. sponge exhibits promising potential as a source of microorganism producing antibacterial compounds, particulary againts MDR (<em>Multidrug-Resistant</em>) strains.</p>2024-03-04T00:00:00+00:00Copyright (c) 2024 Sri Anggarini Rasyid, Sugireng Sugireng, Asni Ramayana Tinahttps://journal2.unusa.ac.id/index.php/IJMLST/article/view/5039Effect of dengue infection on liver profile and hematological parameters2024-03-15T06:04:05+00:00Muthalibu Muhammed Hunaishunaismuthalib@gmail.comShaheed Shiffanasshiffana@gmail.comWalawe Gamage Nimalka Udayanganiunimalka@gmail.com<p>Dengue fever is endemic in Sri Lanka. Liver damage is common in dengue fever. Understanding the pattern change of the liver profile and haematological parameters is crucial for managing dengue patients and minimising liver damage. The purpose of this study was to determine the pattern change in liver profile and the haematological parameters of dengue patients. Sixty apparently healthy volunteers and 169 acute dengue patients aged 20 or older had their blood samples examined. Liver enzymes, albumin, protein, bilirubin, white blood cell count, platelet count, haematocrit, and haemoglobin were measured. There was an increase in aspartate aminotransferase (105.6 IU/L), alanine aminotransferase (82.6 IU/L), and gamma-glutamyl transferase (68.9 IU/L), which were 3.4, 3.9, and 3.2-fold higher, respectively, than those of the control group, suggesting liver injury. Eighty-four percent of patients had raised levels of aspartate aminotransferase, and 74% of patients had elevated levels of alanine aminotransferase, indicating the prominence of aspartate aminotransferase over alanine aminotransferase. The presence of a positive correlation between the levels of aspartate aminotransferase and alanine aminotransferase indicates that the elevation of enzymes is primarily due to a single cause, liver injury. White blood cells and platelet counts were significantly lower. Platelet count demonstrated a negative correlation with both haematocrit and haemoglobin in dengue patients. In conclusion, aspartate aminotransferase was identified as the most significant marker of dengue-associated liver injury, followed by alanine aminotransferase. Low platelet and white blood cell counts were the major unfavourable changes in dengue patients' haematological characteristics.</p>2024-03-15T00:00:00+00:00Copyright (c) 2024 Muthalibu Muhammed Hunais, Shaheed Shiffana, Walawe Gamage Nimalka Udayangani