TY - JOUR AU - Pratiwi, Sari Eka AU - Wahyuningrum, Sri Nuryani AU - Putri, Rachmagreta Perdana AU - Danarto, Danarto AU - Heriyanto, Didik Setyo AU - Arfian, Nur AU - Haryana, Sofia Mubarika AU - Astuti, Indwiani PY - 2022/04/28 Y2 - 2024/03/28 TI - MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia JF - Indonesian Journal of Medical Laboratory Science and Technology JA - IJMLST VL - 4 IS - 1 SE - Articles DO - 10.33086/ijmlst.v4i1.2355 UR - https://journal2.unusa.ac.id/index.php/IJMLST/article/view/2355 SP - 1-9 AB - <p>Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P&lt;0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.</p> ER -