TY - JOUR AU - Riscahyani, Nosy Maria AU - Ekawati, Evy Ratnasari AU - Ngibad, Khoirul PY - 2019/10/18 Y2 - 2024/03/28 TI - IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY JF - Indonesian Journal of Medical Laboratory Science and Technology JA - IJMLST VL - 1 IS - 2 SE - Articles DO - 10.33086/ijmlst.v1i2.1291 UR - https://journal2.unusa.ac.id/index.php/IJMLST/article/view/1291 SP - 58-64 AB - <p>Ascorbic acid mostly comes from vegetables and fruits, especially&nbsp;fresh&nbsp;fruits.&nbsp;Vitamin C is a vitamin that can be formed by several types of plants.&nbsp;One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues.&nbsp;The purpose of this study was to determine the&nbsp;levels of&nbsp;vitamin C contained in papaya using iodimetry and&nbsp;UV-Vis spectrophotometry.&nbsp;The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I<sub>2</sub> until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H<sub>2</sub>SO<sub>4</sub> 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was&nbsp;0.1313%.&nbsp;In conclusion, vitamin C&nbsp;levels&nbsp;analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.</p> ER -